Loading…

Lysozyme functionalized silver nanoclusters as a dual channel optical sensor for the effective determination of glutathione

This article describes the development of a facile and efficient fluorescence sensor for the determination of glutathione (GSH). Presence of the antioxidant glutathione in blood serum is considered as a biomarker for catastrophe like colorectal cancer. Silver nanoclusters with strong fluorescence an...

Full description

Saved in:
Bibliographic Details
Published in:Talanta (Oxford) 2024-09, Vol.277, p.126326, Article 126326
Main Authors: Sam, Sonia, S, Swathy, Girish Kumar, K
Format: Article
Language:English
Subjects:
Citations: Items that this one cites
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:This article describes the development of a facile and efficient fluorescence sensor for the determination of glutathione (GSH). Presence of the antioxidant glutathione in blood serum is considered as a biomarker for catastrophe like colorectal cancer. Silver nanoclusters with strong fluorescence and good water solubility synthesized from relatively cheaper precursors are one of the species very much explored in fluorescence sensors and bioimaging. Here, Chicken egg derived-lysozyme functionalized silver nanoclusters (Lyz AgNCs) with red fluorescence emission has been synthesized and developed to a turn-off fluorescence sensor for GSH through which colorimetric determination is also possible. Due to the ground state ‘Ag–S’ interaction between Lyz AgNCs and GSH, the determination of the analyte is possible from 1.00 × 10−5 M to 1.00 × 10−6 M via fluorimetric and from 9.00 × 10−6 to 8.00 × 10−7 M via spectrophotometric techniques with a limit of detection 2.86 × 10−7 M and 4.76 × 10−7 M, respectively. Selectivity of the sensor has been studied and applicability of the sensor in artificial blood serum samples has been demonstrated. [Display omitted] •Dual channel sensor for the antioxidant-glutathione using red-emitting silver nanoclusters.•Facile one-pot synthesis of stable lysozyme stablilized silver nanoclusters.•Both quenching of fluorescence and color fading of silver nanoclusters on addition of glutathione.•Fluorimetric determination possible from 1.00 × 10−5 M to 1.00 × 10−6 M and spectrophotometric determination from 9.00 × 10−6 to 8.00 × 10−7 M.•Applicability proved via spike recovery analysis in artificial blood serum samples.
ISSN:0039-9140
1873-3573
1873-3573
DOI:10.1016/j.talanta.2024.126326