Staphylococcal enterotoxin B exposed to pregnant rats inhibits the hedgehog signaling pathway in thymic T lymphocytes of the offspring

• Published in: Microbial pathogenesis 2024-07, Vol.192, p.106723, Article 106723
• Main Authors: Zhao, Jia-bao, Fan, Meng-zhu, Shi, Yin-xing, Zhu, Yu-ting, Gao, Shu-xian, Li, Guang-lin, Guan, Jun-chang, Zhou, Ping
• Format: Article
• Language: English
• Subjects: Hedgehog signaling pathway; Staphylococcal enterotoxin B; T lymphocytes; Thymus
• ISSN: 0882-4010; 1096-1208; 1096-1208
• DOI: 10.1016/j.micpath.2024.106723

The Hedgehog (Hh) signaling pathway is involved in T cell differentiation and development and plays a major regulatory part in different stages of T cell development. A previous study by us suggested that prenatal exposure to staphylococcal enterotoxin B (SEB) changed the percentages of T cell subpopulation in the offspring thymus. However, it is unclear whether prenatal SEB exposure impacts the Hh signaling pathway in thymic T cells. In the present study, pregnant rats at gestational day 16 were intravenously injected once with 15 μg SEB, and the thymi of both neonatal and adult offspring rats were aseptically acquired to scrutinize the effects of SEB on the Hh signaling pathway. It firstly found that prenatal SEB exposure clearly caused the increased expression of Shh and Dhh ligands of the Hh signaling pathway in thymus tissue of both neonatal and adult offspring rats, but significantly decreased the expression levels of membrane receptors of Ptch1 and Smo, transcription factor Gli1, as well as target genes of CyclinD1, C-myc, and N-myc in Hh signaling pathway of thymic T cells. These data suggest that prenatal SEB exposure inhibits the Hh signaling pathway in thymic T lymphocytes of the neonatal offspring, and this effect can be maintained in adult offspring via the imprinting effect. •Prenatal exposure to SEB decreased the expression levels of membrane receptors (Ptch1, Smo), transcriptional activator Gli1, target genes (CyclinD1, C-myc, and N-myc) of Hh signaling pathway in thymic T cells of neonatal and adult offspring.•Prenatal exposure to SEB increased the expression levels of ligands (Shh, Dhh) in the thymus tissues.•Prenatal exposure to SEB inhibits the Hh signaling pathway in thymic T lymphocytes of the neonatal offspring, and this effect can be maintained in adult offspring via the imprinting effect.