Degrees of Inflammation in the Treatment of Subglottic Stenosis in a Rabbit Model: Histopathological Assessment of a Novel Bioabsorbable Ultra-high Ductility Magnesium Alloy Stent

Utilizing a novel histopathological scoring system and subglottic stenosis (SGS) rabbit model, we aimed to compare degrees of inflammation and severity of narrowing in the subglottis between two minimally invasive therapeutic modalities: endoscopic balloon dilation (EBD) alone versus EBD with placem...

Full description

Saved in:
Bibliographic Details
Published in:International journal of pediatric otorhinolaryngology 2024-06, Vol.181, p.111994, Article 111994
Main Authors: McCarty, E. Berryhill, Bertoni, Dylan, Patel, Terral, Jaramillo, Couger, Tobey, Allison B.J., Kumta, Prashant N., Chi, David, Mady, Leila J.
Format: Article
Language:English
Subjects:
Absorbable Implants
Alloys
Animals
Biodegradable stents
Dilatation - instrumentation
Disease Models, Animal
Histopathology of tracheal inflammation
Inflammation - pathology
Laryngoscopy
Laryngostenosis - pathology
Laryngostenosis - therapy
Magnesium
Rabbits
Severity of Illness Index
Stents
Subglottic stenosis
Tracheal fibrosis
Tracheal stents
Citations: Items that this one cites
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Utilizing a novel histopathological scoring system and subglottic stenosis (SGS) rabbit model, we aimed to compare degrees of inflammation and severity of narrowing in the subglottis between two minimally invasive therapeutic modalities: endoscopic balloon dilation (EBD) alone versus EBD with placement of a bioabsorbable ultra-high ductility magnesium (UHD-Mg) alloy stent. SGS was induced endoscopically via microsuspension laryngoscopy in 23 New Zealand white rabbits. The control group (n = 11) underwent EBD alone, the study arm (n = 12) underwent EBD with implantation of bioabsorbable UHD-Mg alloy stents. Rabbits were euthanized at 2-, 3-, and 6-weeks after SGS induction, coinciding with wound healing stages. Using Optical Coherence Tomography (OCT), cross-sectional areas of airways were compared to calculate the mean percentage of intraluminal area at sequential time points. A novel histopathological scoring system was used to analyze frozen sections of laryngotracheal complexes. The degree of inflammation was quantified by scoring changes in inflammatory cell infiltration, epithelial ulceration/metaplasia, subepithelial edema/fibrosis, and capillary number/dilation. Univariate analysis was utilized to analyze these markers. We found rabbits implanted with the bioabsorbable UHD-Mg alloy stent had statistically significantly higher scores in categories of hyperplastic change (stents vs controls: 1.48 vs 0.46 p 
ISSN:0165-5876
1872-8464
1872-8464
DOI:10.1016/j.ijporl.2024.111994