Pharmacologic blockade of nicotinic receptors in the suprachiasmatic nucleus increases ovarian atresia and inhibits follicular growth

• Published in: Journal of neuroendocrinology 2024-09, Vol.36 (9), p.e13421-n/a
• Main Authors: Vieyra, Elizabeth, Calderón, Roberto, Linares, Rosa, Rosas, Gabriela, Ramírez, Deyra A., Espinoza, Julieta A., Chaparro, Andrea, Silva, Carlos‐Camilo, Domínguez, Roberto, Morales‐Ledesma, Leticia
• Format: Article
• Language: English
• Subjects: acetylcholine; Acetylcholine receptors (muscarinic); Acetylcholine receptors (nicotinic); Animals; Estrous Cycle - drug effects; Estrus cycle; Female; Follicle-stimulating hormone; Follicles; Follicular Atresia - drug effects; Follicular Atresia - metabolism; follicular development; Folliculogenesis; Gonadotropins; Hypothalamus; Mecamylamine; Mecamylamine - pharmacology; Microinjection; Neurogenesis; Nicotinic Antagonists - pharmacology; nicotinic receptors; Oocytes; Ovarian Follicle - drug effects; Ovarian Follicle - metabolism; Ovaries; Ovulation; Pituitary; Pituitary (anterior); Rats; Rats, Wistar; Receptors, Nicotinic - metabolism; Secretion; Serum levels; Suprachiasmatic nucleus; Suprachiasmatic Nucleus - drug effects; Suprachiasmatic Nucleus - metabolism
• ISSN: 0953-8194; 1365-2826; 1365-2826
• DOI: 10.1111/jne.13421

Reproduction in all mammalian species depends on the growth and maturation of ovarian follicles, that is, folliculogenesis. Follicular development can culminate with the rupture of mature follicles and the consequent expulsion of their oocytes (ovulation) or in atresia, characterized by the arrest of development and eventual degeneration. These processes are regulated by different neuroendocrine signals arising at different hypothalamic nuclei, including the suprachiasmatic nucleus (SCN). In the later, the activation of muscarinic receptors (mAChRs) and nicotinic receptors (nAChRs) by acetylcholine is essential for the regulation of the pre‐ovulatory signals that stimulate the rupture of mature follicles. To evaluate the participation of the nAChRs in the SCN throughout the oestrous cycle in the regulation of the hypothalamic–pituitary–ovarian axis. For this purpose, 90‐day‐old adult female rats in metoestrus, dioestrus, proestrus or oestrus were microinjected into the left‐ or right‐SCN with 0.3 μL of saline solution as vehicle or with 0.225 μg of mecamylamine (Mec), a non‐selective antagonist of the nicotinic receptors, diluted in 0.3 μL of vehicle. The animals were sacrificed when they presented vaginal cornification, indicative of oestrus stage, and the effects of the unilateral pharmacological blockade of the nAChRs in the SCN on follicular development, ovulation and secretion of oestradiol and follicle‐stimulating hormone (FSH) were evaluated. The microinjection of Mec decreased the serum levels of FSH, which resulted in a lower number of growing and healthy follicles and an increase in atresia. The higher percentage of atresia in pre‐ovulatory follicles was related to a decrease in the number of ova shed and abnormalities in oestradiol secretion. We also detected asymmetric responses between the left and right treatments that depended on the stage of the oestrous cycle. The present results allow us to suggest that during all the stages of the oestrous cycle, cholinergic signals that act on the nAChRs in the SCN are pivotal to modulate the secretion of gonadotropins and hence the physiology of the ovaries. Further research is needed to determine if such signals are generated by the cholinergic neurons in the SCN or by cholinergic afferents to the SCN.