Performance of an automated immunoturbidimetric assay for bovine serum amyloid A

Background Acute phase proteins are a group of vital constituents of the innate immune system, which may also serve as circulatory biomarkers of inflammation. The major acute phase protein serum amyloid A (SAA) is a reliable and sensitive biomarker in cows, allowing for rapid detection of inflammato...

Full description

Saved in:
Bibliographic Details
Published in:Veterinary clinical pathology 2024-06, Vol.53 (2), p.229-233
Main Authors: Nielsen, Lise N., Petersen, Mette B., Capion, Nynne, Lundsgaard, Jo F.‐H., Jensen, Asger L.
Format: Article
Language:English
Subjects:
acute phase response
Animals
Automation
biomarker
Biomarkers
Biomarkers - blood
cattle
Cattle - blood
Cattle Diseases - blood
Cattle Diseases - diagnosis
Female
Immunoturbidimetry - methods
Immunoturbidimetry - veterinary
inflammation
Inflammation - blood
Inflammation - diagnosis
Inflammation - veterinary
Inflammatory diseases
Reproducibility of Results
Sensitivity and Specificity
Serum Amyloid A Protein - analysis
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Background Acute phase proteins are a group of vital constituents of the innate immune system, which may also serve as circulatory biomarkers of inflammation. The major acute phase protein serum amyloid A (SAA) is a reliable and sensitive biomarker in cows, allowing for rapid detection of inflammatory disease. A multispecies automated immunoturbidimetric assay (VET‐SAA, Eiken) has been validated for horses, dogs, and cats, and it has been used to measure SAA concentrations in bovine samples. Objectives The aim of the present study was to perform an analytical validation of the VET‐SAA immunoturbidometric assay based on monoclonal antihuman SAA antibodies for the measurement of SAA in clinical samples from cows. Methods and Results The validation included an assessment of imprecision, inaccuracy, and detection limit, as well as an evaluation of the overlap performance, using banked serum from healthy and sick cows with or without inflammatory disease. Intra‐ and interassay variation ranged from 0.91% to 2.9% and 2.5% to 5.8%, respectively. The assay was performed with acceptable accuracy within a clinically relevant range of SAA, although minor signs of inaccuracy were detected. Overlap performance was acceptable, with the VET‐SAA assay able to differentiate between healthy cows and cows with inflammatory and noninflammatory conditions. The automated VET‐SAA assay is considered acceptable for the measurement of SAA in cows.
ISSN:0275-6382
1939-165X
DOI:10.1111/vcp.13355