Utility of green chemistry for feasible spectrofluorometric determination of trifluridine via metal complexation: Application to dosage forms

• Published in: Luminescence (Chichester, England) England), 2024-06, Vol.39 (6), p.e4793-n/a
• Main Authors: Salem, Hesham, Emad, Nadeen, Fares, Joy, Ahmed, Habeba, Kamal, Habiba, Zein, Dina
• Format: Article
• Language: English
• Subjects: Buffers (chemistry); Complex formation; Dosage; Dosage Forms; Drug dosages; Drugs; Fluorescence; Green chemistry; Green Chemistry Technology; Heavy metals; Hydrogen-Ion Concentration; Limit of Detection; Sodium; Sodium dodecyl sulfate; Sodium Dodecyl Sulfate - chemistry; Sodium lauryl sulfate; spectrofluorimetry; Spectrometry, Fluorescence; trifluridine; Trifluridine - analysis; Trifluridine - chemistry; Zinc; Zinc - analysis; Zinc - chemistry
• ISSN: 1522-7235; 1522-7243
• DOI: 10.1002/bio.4793

The goal of the current research was to establish a quick and practical fluorometric technique for trifluridine analysis. The approach relied on the drug's complex formation with the zinc ion to produce a high‐fluorescence product. The fluorescence was further enhanced by adding sodium dodecyl sulfate, and it was observed at 450 nm following excitation at 400 nm. With a determination coefficient of 0.9994, the association between emission intensity and trifluridine concentration was linear between 1 and 125 ng mL−1. The quantitation limit was 0.987 ng mL−1 while 0.333 ng mL−1 was the detection limit. The buffer type, pH and concentration, type of surfactant and concentration, and finally the diluting solvent were among the reaction conditions that were closely examined. With great precision and reliability, the drug in question was quantified using this method in dosage formulations. The proposed method's level of greenness was assessed using two methodologies: the analytical greenness metric (AGREE) and the Green Analytical Procedure Index (GAPI).