Relationships between matrix mineralization, oxidative metabolism, and mitochondrial structure during ATDC5 murine chondroprogenitor cell line differentiation

• Published in: Journal of cellular physiology 2024-08, Vol.239 (8), p.e31285-n/a
• Main Authors: Blank, Kevin, Ekanayake, Derrick, Cooke, Margaret, Bragdon, Beth, Hussein, Amira, Gerstenfeld, Louis
• Format: Article
• Language: English
• Subjects: Actin; Aggrecan; Apoptosis; Cell differentiation; Cell fusion; chondrocyte differentiation; Chondrocytes; Chondrogenesis; Cytoskeleton; Differentiation; Electron transport; Energy metabolism; Extracellular matrix; Fibroblast growth factor 23; Fission; Gene expression; Immunology; Localization; matrix mineralization; Metabolism; Mineralization; Mitochondria; mitochondrial fusion; Mitophagy; Oxidative metabolism; Oxidative phosphorylation; Phosphorylation; Statistical analysis
• ISSN: 0021-9541; 1097-4652; 1097-4652
• DOI: 10.1002/jcp.31285

The mechanistic relationships between the progression of growth chondrocyte differentiation, matrix mineralization, oxidative metabolism, and mitochondria content and structure were examined in the ATDC5 murine chondroprogenitor cell line. The progression of chondrocyte differentiation was associated with a statistically significant (p ≤ 0.05) ~2‐fold increase in oxidative phosphorylation. However, as matrix mineralization progressed, oxidative metabolism decreased. In the absence of mineralization, cartilage extracellular matrix mRNA expression for Col2a1, Aggrecan, and Col10a1 were statistically (p ≤ 0.05) ~2–3‐fold greater than observed in mineralizing cultures. In contrast, BSP and Phex that are associated with promoting matrix mineralization showed statistically (p ≤ 0.05) higher ~2–4 expression, while FGF23 phosphate regulatory factor was significantly lower (~50%) in mineralizing cultures. Cultures induced to differentiate under both nonmineralizing and mineralizing media conditions showed statistically greater basal oxidative metabolism and ATP production. Maximal respiration and spare oxidative capacity were significantly elevated (p ≤ 0.05) in differentiated nonmineralizing cultures compared to those that mineralized. Increased oxidative metabolism was associated with both an increase in mitochondria volume per cell and mitochondria fusion, while mineralization diminished mitochondrial volume and appeared to be associated with fission. Undifferentiated and mineralized cells showed increased mitochondrial co‐localization with the actin cytoskeletal. Examination of proteins associated with mitochondria fission and apoptosis and mitophagy, respectively, showed levels of immunological expression consistent with the increasing fission and apoptosis in mineralizing cultures. These results suggest that chondrocyte differentiation is associated with intracellular structural reorganization, promoting increased mitochondria content and fusion that enables increased oxidative metabolism. Mineralization, however, does not need energy derived from oxidative metabolism; rather, during mineralization, mitochondria appear to undergo fission and mitophagy. In summary, these studies show that as chondrocytes underwent hypertrophic differentiation, they increased oxidative metabolism, but as mineralization proceeds, metabolism decreased. Mitochondria structure also underwent a structural reorganization that was further supportive of their oxidative capacity as the