Development and characterization of polyethylene oxide and guar gum-based hydrogel; a detailed in-vitro analysis of degradation and drug release kinetics

• Published in: International journal of biological macromolecules 2024-07, Vol.273 (Pt 1), p.132824, Article 132824
• Main Authors: Aizaz, Aqsa, Nawaz, Muhammad Haseeb, Ismat, Muhammad Sameet, Zahid, Laiba, Zahid, Sidra, Ahmed, Sheraz, Abbas, Mohamed, Vayalpurayil, Thafasalijyas, Rehman, Muhammad Atiq Ur
• Format: Article
• Language: English
• Subjects: adsorption; angiogenesis; Animals; Anti-Bacterial Agents - chemistry; Anti-Bacterial Agents - pharmacology; Antibacterial; antibacterial properties; body fluids; chorioallantoic membrane; Chorioallantoic Membrane - drug effects; citric acid; crosslinking; Degradation; Drug Carriers - chemistry; Drug Liberation; drugs; electron microscopy; Escherichia; Escherichia coli - drug effects; Fourier transform infrared spectroscopy; Galactans - chemistry; guar gum; Hydrogel film; hydrogels; Hydrogels - chemistry; hydrophilicity; Kinetics; Mannans - chemistry; phosphates; Plant Gums - chemistry; polyethylene glycol; Polyethylene Glycols - chemistry; Release kinetics; rosemary; Staphylococcus; Staphylococcus aureus - drug effects; Sustained
• ISSN: 0141-8130; 1879-0003; 1879-0003
• DOI: 10.1016/j.ijbiomac.2024.132824

Herein, we synthesized hydrogel films from crosslinked polyethylene oxide (PEO) and guar gum (GG) which can offer hydrophilicity, antibacterial efficacy, and neovascularization. This study focuses on synthesis and material/biological characterization of rosemary (RM) and citric acid (CA) loaded PEO/GG hydrogel films. Scanning Electron Microscopy images confirmed the porous structure of the developed hydrogel film matrix (PEO/GG) and the dispersion of RM and CA within it. This porous structure promotes moisture adsorption, cell attachment, proliferation, and tissue layer formation. Fourier Transform Infrared Spectroscopy (FTIR) further validated the crosslinking of the PEO/GG matrix, as confirmed by the appearance of C-O-C linkage in the FTIR spectrum. PEO/GG and PEO/GG/RM/CA revealed similar degradation and release kinetics in Dulbecco's Modified Eagle Medium, Simulated Body Fluid, and Phosphate Buffer Saline (degradation of ∼55 % and release of ∼60 % RM in 168 h.). The developed hydrogel film exhibited a zone of inhibition against Escherichia. coli (2 mm) and Staphylococcus. aureus (9 mm), which can be attributed to the presence of RM in the hydrogel film. Furthermore, incorporating CA in the hydrogel film promoted neovascularization, as confirmed by the Chorioallantoic Membrane Assay. The developed RM and CA-loaded PEO/GG-based hydrogel films offered suitable in-vitro properties that may aid in potential wound healing applications. [Display omitted]