Identification of potential pathogenic genes of intestinal metaplasia based on transcriptomic sequencing and bioinformatics analysis

To explore the potential pathogenic genes of intestinal metaplasia. Twenty-one patients with intestinal metaplasia admitted to the Department of Gastroenterology at the Second Affiliated Hospital of Anhui University of Chinese Medicine from January, 2022 to June, 2022, and 21 healthy subjects underg...

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Published in:Nan fang yi ke da xue xue bao = Journal of Southern Medical University 2024-05, Vol.44 (5), p.941
Main Authors: Pei, B, Zhang, Y, Wei, S, Mei, Y, Song, B, Dong, G, Wen, Z, Li, X
Format: Article
Language:Chinese
Subjects:
Computational Biology - methods
Fatty Acid-Binding Proteins - genetics
Female
Gastric Mucosa - metabolism
Gastric Mucosa - pathology
Gene Expression Profiling
Homeodomain Proteins - genetics
Humans
Intestines - pathology
Male
Metaplasia - genetics
Mucin-2 - genetics
Mucin-2 - metabolism
RNA, Messenger - genetics
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Summary:To explore the potential pathogenic genes of intestinal metaplasia. Twenty-one patients with intestinal metaplasia admitted to the Department of Gastroenterology at the Second Affiliated Hospital of Anhui University of Chinese Medicine from January, 2022 to June, 2022, and 21 healthy subjects undergoing gastroscopic examination during the same period were enrolled in this study. All the participants underwent gastroscopy and pathological examination, and gastric tissue samples were collected for transcriptome sequencing to screen for differentially expressed genes (DEGs). The biological functions of the DEGs were analyzed using bioinformatics analysis, and qRT-PCR was used to validate the results. Transcriptomic sequencing identified a total of 1373 DEGs, including 827 upregulated and 546 downregulated ones. The top 6 upregulated genes ( , , , , , and ), ranked based on their significance and average expression level, were selected for validation, and qRT-PCR showed significant upregulation of their mRNAs in
ISSN:1673-4254
DOI:10.12122/j.issn.1673-4254.2024.05.16