Characterization and antibacterial evaluation of Eco-friendly silver nanoparticles synthesized by halophilic Streptomyces rochei SSCM102 isolated from mangrove sediment

• Published in: Molecular biology reports 2024-12, Vol.51 (1), p.730
• Main Authors: Nainangu, Prasannabalaji, Mothilal, Sudhalakshmi Nattanmai, Subramanian, Kumaran, Thanigaimalai, Murugan, Kandasamy, Rajesh, Srinivasan, Guru Prasad, Gopal, Suresh, Shaik, Mohammed Rafi, Kari, Zulhisyam Abdul, Guru, Ajay, Antonyraj, Anahas Perianaika Matharasi
• Format: Article
• Language: English
• Subjects: Actinomycetes; Animal Anatomy; Animal Biochemistry; Anti-Bacterial Agents - chemistry; Anti-Bacterial Agents - pharmacology; Antimicrobial agents; Antimicrobial resistance; Bacteria - drug effects; Bioactive compounds; Biodiversity hot spots; Biomedical and Life Sciences; Drug development; Geologic Sediments - microbiology; Green Chemistry Technology - methods; Histology; India; Life Sciences; Medical research; Metal Nanoparticles - chemistry; Microbial Sensitivity Tests; Morphology; Nanoparticles; Original Article; Silver; Silver - chemistry; Silver - pharmacology; Spectroscopy, Fourier Transform Infrared - methods; Streptomyces - metabolism; Streptomyces rochei; Toxicity
• ISSN: 0301-4851; 1573-4978; 1573-4978
• DOI: 10.1007/s11033-024-09666-4

Background Antimicrobial resistance has surged due to widespread antimicrobial drug use, prompting interest in biosynthesizing nanoparticles from marine-derived actinomycetes extracellular metabolites, valued for their diverse bioactive compounds. This approach holds promise for addressing the urgent need for novel antimicrobial agents. The current study aimed to characterize novel bioactive compounds from unexplored biodiversity hotspots, halophilic Streptomyces sp. isolated from mangrove sediment in the Pichavaram region, India. Methods and results Streptomyces rochei SSCM102 was conclusively identified through morphological and molecular characterization. Synthesis of silver nanoparticles (AgNPs) from Streptomyces rochei SSCM102 was characterized using various techniques, including UV-Vis, XRD, SEM, EDX, and FT-IR. The UV-Vis spectrum of the reduced AgNPs exhibited a prominent peak at 380 nm, confirming the AgNPs. The UV-Vis spectrum confirmed the synthesis of AgNP, and SEM analysis revealed a cubic morphology with sizes ranging from 11 to 21 nm. The FTIR spectrum demonstrated a shift in frequency widths between 626 cm -1 and 3432 cm -1 . The EDX analysis substantiated the presence of metallic silver, evident from a strong band at 1.44 keV. The synthesized AgNPs exhibited antibacterial efficacy against human pathogens Escherichia coli (64 ± 0.32 µg/ml), Klebsiella pneumoniae (32 ± 0.16 µg/ml), and Pseudomonas aeruginosa (16 ± 0.08 µg/ml) by MIC and MBC values of 128 ± 0.64 (µg/ml), 64 ± 0.32 (µg/ml) and 32 ± 0.16 (µg/ml), respectively. Additionally, at a concentration of 400 µg/ml, the AgNPs displayed a 72% inhibition of DPPH radicals, indicating notable antioxidant capacity. The LC 50 value of 130 µg/mL indicates that the green-synthesized AgNPs have lower toxicity by Brine Shrimp Larvae assay. Conclusion The study’s novel approach to synthesizing eco-friendly silver nanoparticles using Halophilic Streptomyces rochei SSCM102 contributes significantly to the field of biomedical research and drug development. By demonstrating potent antibacterial properties and aligning with sustainability goals, these nanoparticles offer promising avenues for novel antibacterial therapies.