Effects of different organic substrate compositions on the decontamination of aged PAH-polluted soils through outdoor co-composting

• Published in: Chemosphere (Oxford) 2024-08, Vol.362, p.142580, Article 142580
• Main Authors: Němcová, Kateřina, Lhotský, Ondřej, Stavělová, Monika, Komárek, Michael, Semerád, Jaroslav, Filipová, Alena, Najmanová, Petra, Cajthaml, Tomáš
• Format: Article
• Language: English
• Subjects: Bioavailability; Bioremediation; Co-composting; Organic substrate; Polycyclic aromatic hydrocarbons; Semi-pilot
• ISSN: 0045-6535; 1879-1298; 1879-1298
• DOI: 10.1016/j.chemosphere.2024.142580

The effects of different organic substrate compositions on the efficiency of outdoor co-composting as a bioremediation technology for decontaminating soil polluted by polycyclic aromatic hydrocarbons (PAHs) were investigated. Four different substrate mixtures and two different aged PAH-contaminated soils were used in a semi-pilot-scale experiment that lasted nearly 700 days. The two soils (A and B) differed concerning both the initial concentrations of the Ʃ16 US EPA PAHs (5926 vs. 369 mg kg−1, respectively) and the type of predominant PAH group by molecular weight. The experiments revealed that while the composition of the organic substrate had an impact on the rate of PAH degradation, it did not significantly influence the final extent of PAH degradation. Notably, the organic substrate consisting of green waste and wood chips (GW) was found to facilitate the most rapid rate of PAH degradation (first-order rate constant k = 0.033 ± 0.000 d−1 with soil A over the initial 42 days of the experiment and k = 0.036 ± 0.000 d−1 with soil B over the initial 56 days). Despite the differences in organic substrate compositions and types of soil being treated, PAH degradation levels exceeded at least 95% in all the treatments after more than 680 days of co-composting. Regardless of the composition, the removal of low- and medium- molecular-weight (2-4 rings) PAHs was nearly complete by the end of the experiment. Furthermore, high-molecular-weight PAHs (5 rings and more) were significantly degraded during co-composting, with reductions ranging from 54% to 79% in soil A and from 59% to 68% in soil B. All composts were dominated by Proteobacteria, Firmicutes, and Actinobacteria, with significant differences in abundance between soils. Genera with PAH degradation potentials were detected in all samples. The results of a battery of toxicity tests showed that there was almost no toxicity associated with the final composts. [Display omitted] •Co-composting is efficient in decontaminating aged PAH-polluted soils.•Organic substrate compositions do not influence the extent of PAH degradation.•Green waste and wood chips promote the fastest PAH degradation compared to others.•The whole degrading community rather than a particular organism is important.•PAH levels exceeded the predicted bioavailable part, even without added surfactants.