Bifunctional naringenin-laden gelatin methacryloyl scaffolds with osteogenic and anti-inflammatory properties

To fabricate and characterize an innovative gelatin methacryloyl/GelMA electrospun scaffold containing the citrus flavonoid naringenin/NA with osteogenic and anti-inflammatory properties. GelMA scaffolds (15 % w/v) containing 0/Control, 5, 10, or 20 % of NA w/w were obtained via electrospinning. The...

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Bibliographic Details
Published in:Dental materials 2024-09, Vol.40 (9), p.1353-1363
Main Authors: Cardoso, Lais M., de Carvalho, Ana Beatriz G., Anselmi, Caroline, Mahmoud, Abdel H., Dal-Fabbro, Renan, Basso, Fernanda G., Bottino, Marco C.
Format: Article
Language:English
Subjects:
Alkaline Phosphatase - metabolism
Anti-Inflammatory Agents - pharmacology
Bone
Cell Adhesion - drug effects
Cell Proliferation - drug effects
Cells, Cultured
Core Binding Factor Alpha 1 Subunit
Electrospinning
Flavanones - chemistry
Flavanones - pharmacology
Flavonoids
Gelatin - chemistry
Gelatin methacryloyl
Humans
Inflammation
Mesenchymal Stem Cells - drug effects
Methacrylates - chemistry
Osteogenesis - drug effects
Regeneration
Tissue Engineering
Tissue Scaffolds - chemistry
Tumor Necrosis Factor-alpha
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Summary:To fabricate and characterize an innovative gelatin methacryloyl/GelMA electrospun scaffold containing the citrus flavonoid naringenin/NA with osteogenic and anti-inflammatory properties. GelMA scaffolds (15 % w/v) containing 0/Control, 5, 10, or 20 % of NA w/w were obtained via electrospinning. The chemical composition, fiber morphology/diameter, swelling/degradation profile, and NA release were investigated. Cytotoxicity, cell proliferation, adhesion and spreading, total protein/TP production, alkaline phosphatase/ALP activity, osteogenic genes expression (OCN, OPN, RUNX2), and mineralized nodules deposition/MND with human alveolar bone-derived mesenchymal stem cells (aBMSCs) seeded on the scaffolds were assessed. Moreover, aBMSCs seeded on the scaffolds and stimulated with tumor necrosis factor-alpha/TNF-α were submitted to collagen, nitric oxide/NO, interleukin/IL-1α, and IL-6 production assessment. Data were analyzed using ANOVA and t-student/post-hoc tests (α = 5 %). NA-laden scaffolds presented increased fiber diameter, lower swelling capacity, and faster degradation profile over 28 days (p 
ISSN:0109-5641
1879-0097
1879-0097
DOI:10.1016/j.dental.2024.06.019