Metagenomics insight into Puga geothermal geyser located in Himalayan Geothermal Belt (Trans-Himalayan Plateau) Ladakh, India

• Published in: Brazilian journal of microbiology 2024-09, Vol.55 (3), p.2321-2334
• Main Authors: Kumari, Shalini, Choudhary, Geetanjli, Anu, Kumari, Devi, Sarita
• Format: Article
• Language: English
• Subjects: Anthropogenic factors; Archaea - classification; Archaea - genetics; Archaea - isolation & purification; Bacteria; Bacteria - classification; Bacteria - genetics; Bacteria - isolation & purification; Biomedical and Life Sciences; Environment and Biodiversity - Research Paper; Food Microbiology; Gene sequencing; Genomic analysis; Geysers; Hot Springs - microbiology; India; Life Sciences; Medical Microbiology; Metagenome; Metagenomics; Microbial Ecology; Microbial Genetics and Genomics; Microbiology; Microbiota; Microorganisms; Mycology; Phylogeny; Whole genome sequencing
• ISSN: 1517-8382; 1678-4405; 1678-4405
• DOI: 10.1007/s42770-024-01408-9

Puga geothermal geyser and surrounding area, located in the Himalayan Geothermal Belt of the Trans-Himalayan Plateau in Ladakh, India, are very geographically isolated and considered pristine and free of anthropogenic activities. In this study, we have conducted the first metagenomic investigation of the microbes in and around the geyser. The whole genome sequencing analysis showed the presence of a total of 44.8%, 39.7% and 41.4% bacterial phyla in the PugW, PugS, and PugSo samples respectively, 8.6% of archaeal phyla (in all the samples), unclassified (derived from other sequences, PugW: 27.6%, PugS: 27.6%, and PugSo: 15.5%) and unclassified (derived from bacteria, PugW: 12%, PugS: 13.8%, and PugSo: 13.8%). The majority of archaeal sequences were linked to Euryarchaeota (2.84%) while the majority of the bacterial communities that predominated in most geothermal locations were linked to Pseudomonadota (67.14%) and Bacteroidota (12.52%). The abundant bacterial strains at the species level included Dechloromonas aromatica , Acinetobacter baumannii , and Arcobacter butzleri , in all the samples while the most abundant archaeal species were Methanosaeta thermophile, Methanoregula boonei, and Methanosarcina berkeri. Further, this geothermal geyser metagenome has a large number of unique sequences linked to unidentified and unclassified lineages, suggesting a potential source for novel species of microbes and their products. The present study which only examined one of the many geothermal geysers and springs in the Puga geothermal area, should be regarded as a preliminary investigation of the microbiota that live in the geothermal springs on these remote areas. These findings suggest that further investigations should be undertaken to characterize the ecosystems of the Puga geothermal area, which serve as a repository for unidentified microbial lineages.