Effect of hormonal treatment on artificial propagation, spawning performance and embryonic development of striped snakehead Channa striata (Bloch, 1793)

• Published in: Animal reproduction science 2024-08, Vol.267, p.107521, Article 107521
• Main Authors: Rabiul Awal, Md, Pervin, Rubaiya, Ashikur Rahman, Md, Bhadra, Anuradha, Mahmud, Yahia, Tanu, Mohosena Begum, Parvez, Imran
• Format: Article
• Language: English
• Subjects: Animals; Aquaculture - methods; Buserelin; Buserelin - administration & dosage; Buserelin - pharmacology; Carp pituitary extract; Carps - physiology; Embryonic development; Embryonic Development - drug effects; Female; Fishes - physiology; Male; Perciformes - physiology; Pituitary Hormones - pharmacology; Reproduction - drug effects; Spawning induction
• ISSN: 0378-4320; 1873-2232; 1873-2232
• DOI: 10.1016/j.anireprosci.2024.107521

Present study describes the spawning induction of striped Snakehead, Channa striata using carp pituitary extract (CPE) and LH-RH agonist i.e. Buserelin (Glp-His-Trp-Ser-Tyr-Ser-tBu-Leu-Arg-Pro-NHEt). Total four treatments were designed under both hormones trail and treated as control group, T1, T2, and T3 with three replications of each treatment. While breeders under all hormone treatments showed spawning performances, no spawning performance was observed in control group. Latency time after hormonal treatment was lowest (20–24 hrs) in case of CPE than Buserelin (25–30 hrs). Regarding to CPE, spawning, fertilization and hatching rate were higher with the increasing doses of CPE in different treatments. The highest mean ± Standard deviation spawning, fertilization and hatching rate were 85.60±8.58 %, 79.38±4.89 % and 64.33±6.60 % respectively in T3 where dose of CPE was 80 mg kg−1. Similarly, in case of Buserelin hormone highest spawning rate was found in T3 (80.61±5.59) where dose of Buserelin was 0.80 µg kg−1 body weight. Fertilization rate was on the level 48.57±5.99, 70.62±5.33 and 90.32±4.79 respectively for T1, T2, and T3.Whilst, hatching rate was found 20.81±4.91, 37.11±4.50 and 61.33±6.61 in T1, T2, and T3 treatments respectively. However, T3 exhibited best performance regarding spawning, fertilization and hatching rate which were significantly higher than other two treatments.The current study revealed that spawning induction using carp pituitary extract and Buserelin is effective and might be useful for artificial breeding of Channa striata. Regarding to dose application i.e. 80 mg kg−1 of CPE and 0.80 µg kg−1 of Buserelin may be successfully applied to ovulation stimulation of Channa striata. •Carp pituitary extract (CPE) and Buserelin were efficient in spawning induction.•Spawning performances were higher with the increasing doses of hormones.•Most effective doses were 80 mg kg−1 and 0.80 µg kg−1 for CPE and Buserelin.•The result will be helpful to design induced breeding of Channa striata.