Loading…
Lipid and sugar metabolism play an essential role in pollen development and male sterility: a case analysis in Brassica napus
Aims The genic male sterility (GMS) system is an important strategy for generating heterosis in plants. To better understand the essential role of lipid and sugar metabolism and to identify additional candidates for pollen development and male sterility, transcriptome and metabolome analysis of a GM...
Saved in:
| Published in: | Physiologia plantarum 2024-05, Vol.176 (3), p.e14394-n/a |
|---|---|
| Main Authors: | , , , , , , , , , , |
| Format: | Article |
| Language: | English |
| Subjects: | |
| Citations: | Items that this one cites |
| Online Access: | Get full text |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| cited_by | |
|---|---|
| cites | cdi_FETCH-LOGICAL-c2434-8dde19ce408f7697911a59904a625dfd196e3204bb1ee369df353521155966493 |
| container_end_page | n/a |
| container_issue | 3 |
| container_start_page | e14394 |
| container_title | Physiologia plantarum |
| container_volume | 176 |
| creator | Xiao, Lijing Wang, Xianya Jiang, Yingfen Ye, Botao Yu, Kunjiang Wang, Qian Yang, Xu Zhang, Jinze Ouyang, Qingjing Jin, Hairui Tian, Entang |
| description | Aims
The genic male sterility (GMS) system is an important strategy for generating heterosis in plants. To better understand the essential role of lipid and sugar metabolism and to identify additional candidates for pollen development and male sterility, transcriptome and metabolome analysis of a GMS line of 1205AB in B. napus was used as a case study.
Data resources generated
To characterize the GMS system, the transcriptome and metabolome profiles were generated for 24 samples and 48 samples of 1205AB in B. napus, respectively. Transcriptome analysis yielded a total of 156.52 Gb of clean data and revealed the expression levels of 109,541 genes and 8,501 novel genes. In addition, a total of 1,353 metabolites were detected in the metabolomic analysis, including 784 in positive ion mode and 569 in negative ion mode.
Key results
A total of 15,635 differentially expressed genes (DEGs) and 83 differential metabolites (DMs) were identified from different comparison groups, most of which were involved in lipid and sugar metabolism. The combination of transcriptome and metabolome analysis revealed 49 orthologous GMS genes related to lipid metabolism and 46 orthologous GMS genes related to sugar metabolism, as well as 45 novel genes.
Utility of the resource
The transcriptome and metabolome profiles and their analysis provide useful reference data for the future discovery of additional GMS genes and the development of more robust male sterility breeding systems for use in the production of plant hybrids. |
| doi_str_mv | 10.1111/ppl.14394 |
| format | article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_3070803164</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>3070803164</sourcerecordid><originalsourceid>FETCH-LOGICAL-c2434-8dde19ce408f7697911a59904a625dfd196e3204bb1ee369df353521155966493</originalsourceid><addsrcrecordid>eNp1kcFO3DAQhq2qCLaUQ1-gstQLPQQ8seONuQGiLdJK5dCeI288qYycxHiSVjn03TG7lANSffHh__xZMz9jH0CcQT7nMYYzUNKoN2wF0phCikq9ZSshJBRGwvqIvSO6FwK0hvKQHcm6NqqS1Yr93fjoHbeD4zT_son3ONntGDz1PAa75IQjEQ6Tt4GnMSD3A49jCDhwh78xjLHP6c7Q2xzThMkHPy0X3PLWEubIhoU8Pb28SpbIt5YPNs70nh10NhCePN_H7OeXmx_X34rN96-315eboi2VVEXtHIJpUYm6W2uzNgC2MkYoq8vKdQ6MRlkKtd0CotTGdTIPVwJUldFaGXnMTvfemMaHGWlqek8thmAHHGdqpFiLOi9Lq4x-eoXej3PKE-woyL8KBZn6vKfaNBIl7JqYfG_T0oBonjppcifNrpPMfnw2ztse3Qv5r4QMnO-BPz7g8n9Tc3e32SsfAc8xlUc</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>3071990041</pqid></control><display><type>article</type><title>Lipid and sugar metabolism play an essential role in pollen development and male sterility: a case analysis in Brassica napus</title><source>Wiley</source><creator>Xiao, Lijing ; Wang, Xianya ; Jiang, Yingfen ; Ye, Botao ; Yu, Kunjiang ; Wang, Qian ; Yang, Xu ; Zhang, Jinze ; Ouyang, Qingjing ; Jin, Hairui ; Tian, Entang</creator><creatorcontrib>Xiao, Lijing ; Wang, Xianya ; Jiang, Yingfen ; Ye, Botao ; Yu, Kunjiang ; Wang, Qian ; Yang, Xu ; Zhang, Jinze ; Ouyang, Qingjing ; Jin, Hairui ; Tian, Entang</creatorcontrib><description>Aims
The genic male sterility (GMS) system is an important strategy for generating heterosis in plants. To better understand the essential role of lipid and sugar metabolism and to identify additional candidates for pollen development and male sterility, transcriptome and metabolome analysis of a GMS line of 1205AB in B. napus was used as a case study.
Data resources generated
To characterize the GMS system, the transcriptome and metabolome profiles were generated for 24 samples and 48 samples of 1205AB in B. napus, respectively. Transcriptome analysis yielded a total of 156.52 Gb of clean data and revealed the expression levels of 109,541 genes and 8,501 novel genes. In addition, a total of 1,353 metabolites were detected in the metabolomic analysis, including 784 in positive ion mode and 569 in negative ion mode.
Key results
A total of 15,635 differentially expressed genes (DEGs) and 83 differential metabolites (DMs) were identified from different comparison groups, most of which were involved in lipid and sugar metabolism. The combination of transcriptome and metabolome analysis revealed 49 orthologous GMS genes related to lipid metabolism and 46 orthologous GMS genes related to sugar metabolism, as well as 45 novel genes.
Utility of the resource
The transcriptome and metabolome profiles and their analysis provide useful reference data for the future discovery of additional GMS genes and the development of more robust male sterility breeding systems for use in the production of plant hybrids.</description><identifier>ISSN: 0031-9317</identifier><identifier>ISSN: 1399-3054</identifier><identifier>EISSN: 1399-3054</identifier><identifier>DOI: 10.1111/ppl.14394</identifier><identifier>PMID: 38894535</identifier><language>eng</language><publisher>Oxford, UK: Blackwell Publishing Ltd</publisher><subject>Brassica napus - genetics ; Brassica napus - growth & development ; Brassica napus - metabolism ; Brassica napus - physiology ; Carbohydrate Metabolism - genetics ; Gene Expression Profiling ; Gene Expression Regulation, Plant ; Genes ; Heterosis ; Hybrids ; Lipid metabolism ; Lipid Metabolism - genetics ; Lipids ; Male sterility ; Males ; Metabolism ; Metabolites ; Metabolome - genetics ; Metabolomics ; Negative ions ; Plant Infertility - genetics ; Plant Infertility - physiology ; Pollen ; Pollen - genetics ; Pollen - growth & development ; Pollen - metabolism ; Pollen - physiology ; Positive ions ; Sugar ; Sugars - metabolism ; Transcriptome - genetics ; Transcriptomes</subject><ispartof>Physiologia plantarum, 2024-05, Vol.176 (3), p.e14394-n/a</ispartof><rights>2024 Scandinavian Plant Physiology Society.</rights><rights>2024 Scandinavian Plant Physiology Society</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><cites>FETCH-LOGICAL-c2434-8dde19ce408f7697911a59904a625dfd196e3204bb1ee369df353521155966493</cites><orcidid>0000-0002-9039-3666</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,777,781,27905,27906</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/38894535$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Xiao, Lijing</creatorcontrib><creatorcontrib>Wang, Xianya</creatorcontrib><creatorcontrib>Jiang, Yingfen</creatorcontrib><creatorcontrib>Ye, Botao</creatorcontrib><creatorcontrib>Yu, Kunjiang</creatorcontrib><creatorcontrib>Wang, Qian</creatorcontrib><creatorcontrib>Yang, Xu</creatorcontrib><creatorcontrib>Zhang, Jinze</creatorcontrib><creatorcontrib>Ouyang, Qingjing</creatorcontrib><creatorcontrib>Jin, Hairui</creatorcontrib><creatorcontrib>Tian, Entang</creatorcontrib><title>Lipid and sugar metabolism play an essential role in pollen development and male sterility: a case analysis in Brassica napus</title><title>Physiologia plantarum</title><addtitle>Physiol Plant</addtitle><description>Aims
The genic male sterility (GMS) system is an important strategy for generating heterosis in plants. To better understand the essential role of lipid and sugar metabolism and to identify additional candidates for pollen development and male sterility, transcriptome and metabolome analysis of a GMS line of 1205AB in B. napus was used as a case study.
Data resources generated
To characterize the GMS system, the transcriptome and metabolome profiles were generated for 24 samples and 48 samples of 1205AB in B. napus, respectively. Transcriptome analysis yielded a total of 156.52 Gb of clean data and revealed the expression levels of 109,541 genes and 8,501 novel genes. In addition, a total of 1,353 metabolites were detected in the metabolomic analysis, including 784 in positive ion mode and 569 in negative ion mode.
Key results
A total of 15,635 differentially expressed genes (DEGs) and 83 differential metabolites (DMs) were identified from different comparison groups, most of which were involved in lipid and sugar metabolism. The combination of transcriptome and metabolome analysis revealed 49 orthologous GMS genes related to lipid metabolism and 46 orthologous GMS genes related to sugar metabolism, as well as 45 novel genes.
Utility of the resource
The transcriptome and metabolome profiles and their analysis provide useful reference data for the future discovery of additional GMS genes and the development of more robust male sterility breeding systems for use in the production of plant hybrids.</description><subject>Brassica napus - genetics</subject><subject>Brassica napus - growth & development</subject><subject>Brassica napus - metabolism</subject><subject>Brassica napus - physiology</subject><subject>Carbohydrate Metabolism - genetics</subject><subject>Gene Expression Profiling</subject><subject>Gene Expression Regulation, Plant</subject><subject>Genes</subject><subject>Heterosis</subject><subject>Hybrids</subject><subject>Lipid metabolism</subject><subject>Lipid Metabolism - genetics</subject><subject>Lipids</subject><subject>Male sterility</subject><subject>Males</subject><subject>Metabolism</subject><subject>Metabolites</subject><subject>Metabolome - genetics</subject><subject>Metabolomics</subject><subject>Negative ions</subject><subject>Plant Infertility - genetics</subject><subject>Plant Infertility - physiology</subject><subject>Pollen</subject><subject>Pollen - genetics</subject><subject>Pollen - growth & development</subject><subject>Pollen - metabolism</subject><subject>Pollen - physiology</subject><subject>Positive ions</subject><subject>Sugar</subject><subject>Sugars - metabolism</subject><subject>Transcriptome - genetics</subject><subject>Transcriptomes</subject><issn>0031-9317</issn><issn>1399-3054</issn><issn>1399-3054</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2024</creationdate><recordtype>article</recordtype><recordid>eNp1kcFO3DAQhq2qCLaUQ1-gstQLPQQ8seONuQGiLdJK5dCeI288qYycxHiSVjn03TG7lANSffHh__xZMz9jH0CcQT7nMYYzUNKoN2wF0phCikq9ZSshJBRGwvqIvSO6FwK0hvKQHcm6NqqS1Yr93fjoHbeD4zT_son3ONntGDz1PAa75IQjEQ6Tt4GnMSD3A49jCDhwh78xjLHP6c7Q2xzThMkHPy0X3PLWEubIhoU8Pb28SpbIt5YPNs70nh10NhCePN_H7OeXmx_X34rN96-315eboi2VVEXtHIJpUYm6W2uzNgC2MkYoq8vKdQ6MRlkKtd0CotTGdTIPVwJUldFaGXnMTvfemMaHGWlqek8thmAHHGdqpFiLOi9Lq4x-eoXej3PKE-woyL8KBZn6vKfaNBIl7JqYfG_T0oBonjppcifNrpPMfnw2ztse3Qv5r4QMnO-BPz7g8n9Tc3e32SsfAc8xlUc</recordid><startdate>202405</startdate><enddate>202405</enddate><creator>Xiao, Lijing</creator><creator>Wang, Xianya</creator><creator>Jiang, Yingfen</creator><creator>Ye, Botao</creator><creator>Yu, Kunjiang</creator><creator>Wang, Qian</creator><creator>Yang, Xu</creator><creator>Zhang, Jinze</creator><creator>Ouyang, Qingjing</creator><creator>Jin, Hairui</creator><creator>Tian, Entang</creator><general>Blackwell Publishing Ltd</general><general>Wiley Subscription Services, Inc</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7SN</scope><scope>7ST</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>P64</scope><scope>RC3</scope><scope>SOI</scope><scope>7X8</scope><orcidid>https://orcid.org/0000-0002-9039-3666</orcidid></search><sort><creationdate>202405</creationdate><title>Lipid and sugar metabolism play an essential role in pollen development and male sterility: a case analysis in Brassica napus</title><author>Xiao, Lijing ; Wang, Xianya ; Jiang, Yingfen ; Ye, Botao ; Yu, Kunjiang ; Wang, Qian ; Yang, Xu ; Zhang, Jinze ; Ouyang, Qingjing ; Jin, Hairui ; Tian, Entang</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c2434-8dde19ce408f7697911a59904a625dfd196e3204bb1ee369df353521155966493</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2024</creationdate><topic>Brassica napus - genetics</topic><topic>Brassica napus - growth & development</topic><topic>Brassica napus - metabolism</topic><topic>Brassica napus - physiology</topic><topic>Carbohydrate Metabolism - genetics</topic><topic>Gene Expression Profiling</topic><topic>Gene Expression Regulation, Plant</topic><topic>Genes</topic><topic>Heterosis</topic><topic>Hybrids</topic><topic>Lipid metabolism</topic><topic>Lipid Metabolism - genetics</topic><topic>Lipids</topic><topic>Male sterility</topic><topic>Males</topic><topic>Metabolism</topic><topic>Metabolites</topic><topic>Metabolome - genetics</topic><topic>Metabolomics</topic><topic>Negative ions</topic><topic>Plant Infertility - genetics</topic><topic>Plant Infertility - physiology</topic><topic>Pollen</topic><topic>Pollen - genetics</topic><topic>Pollen - growth & development</topic><topic>Pollen - metabolism</topic><topic>Pollen - physiology</topic><topic>Positive ions</topic><topic>Sugar</topic><topic>Sugars - metabolism</topic><topic>Transcriptome - genetics</topic><topic>Transcriptomes</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Xiao, Lijing</creatorcontrib><creatorcontrib>Wang, Xianya</creatorcontrib><creatorcontrib>Jiang, Yingfen</creatorcontrib><creatorcontrib>Ye, Botao</creatorcontrib><creatorcontrib>Yu, Kunjiang</creatorcontrib><creatorcontrib>Wang, Qian</creatorcontrib><creatorcontrib>Yang, Xu</creatorcontrib><creatorcontrib>Zhang, Jinze</creatorcontrib><creatorcontrib>Ouyang, Qingjing</creatorcontrib><creatorcontrib>Jin, Hairui</creatorcontrib><creatorcontrib>Tian, Entang</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Ecology Abstracts</collection><collection>Environment Abstracts</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><collection>Environment Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Physiologia plantarum</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Xiao, Lijing</au><au>Wang, Xianya</au><au>Jiang, Yingfen</au><au>Ye, Botao</au><au>Yu, Kunjiang</au><au>Wang, Qian</au><au>Yang, Xu</au><au>Zhang, Jinze</au><au>Ouyang, Qingjing</au><au>Jin, Hairui</au><au>Tian, Entang</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Lipid and sugar metabolism play an essential role in pollen development and male sterility: a case analysis in Brassica napus</atitle><jtitle>Physiologia plantarum</jtitle><addtitle>Physiol Plant</addtitle><date>2024-05</date><risdate>2024</risdate><volume>176</volume><issue>3</issue><spage>e14394</spage><epage>n/a</epage><pages>e14394-n/a</pages><issn>0031-9317</issn><issn>1399-3054</issn><eissn>1399-3054</eissn><abstract>Aims
The genic male sterility (GMS) system is an important strategy for generating heterosis in plants. To better understand the essential role of lipid and sugar metabolism and to identify additional candidates for pollen development and male sterility, transcriptome and metabolome analysis of a GMS line of 1205AB in B. napus was used as a case study.
Data resources generated
To characterize the GMS system, the transcriptome and metabolome profiles were generated for 24 samples and 48 samples of 1205AB in B. napus, respectively. Transcriptome analysis yielded a total of 156.52 Gb of clean data and revealed the expression levels of 109,541 genes and 8,501 novel genes. In addition, a total of 1,353 metabolites were detected in the metabolomic analysis, including 784 in positive ion mode and 569 in negative ion mode.
Key results
A total of 15,635 differentially expressed genes (DEGs) and 83 differential metabolites (DMs) were identified from different comparison groups, most of which were involved in lipid and sugar metabolism. The combination of transcriptome and metabolome analysis revealed 49 orthologous GMS genes related to lipid metabolism and 46 orthologous GMS genes related to sugar metabolism, as well as 45 novel genes.
Utility of the resource
The transcriptome and metabolome profiles and their analysis provide useful reference data for the future discovery of additional GMS genes and the development of more robust male sterility breeding systems for use in the production of plant hybrids.</abstract><cop>Oxford, UK</cop><pub>Blackwell Publishing Ltd</pub><pmid>38894535</pmid><doi>10.1111/ppl.14394</doi><tpages>13</tpages><orcidid>https://orcid.org/0000-0002-9039-3666</orcidid></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0031-9317 |
| ispartof | Physiologia plantarum, 2024-05, Vol.176 (3), p.e14394-n/a |
| issn | 0031-9317 1399-3054 1399-3054 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_3070803164 |
| source | Wiley |
| subjects | Brassica napus - genetics Brassica napus - growth & development Brassica napus - metabolism Brassica napus - physiology Carbohydrate Metabolism - genetics Gene Expression Profiling Gene Expression Regulation, Plant Genes Heterosis Hybrids Lipid metabolism Lipid Metabolism - genetics Lipids Male sterility Males Metabolism Metabolites Metabolome - genetics Metabolomics Negative ions Plant Infertility - genetics Plant Infertility - physiology Pollen Pollen - genetics Pollen - growth & development Pollen - metabolism Pollen - physiology Positive ions Sugar Sugars - metabolism Transcriptome - genetics Transcriptomes |
| title | Lipid and sugar metabolism play an essential role in pollen development and male sterility: a case analysis in Brassica napus |
| url | http://sfxeu10.hosted.exlibrisgroup.com/loughborough?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-19T22%3A14%3A41IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Lipid%20and%20sugar%20metabolism%20play%20an%20essential%20role%20in%20pollen%20development%20and%20male%20sterility:%20a%20case%20analysis%20in%20Brassica%20napus&rft.jtitle=Physiologia%20plantarum&rft.au=Xiao,%20Lijing&rft.date=2024-05&rft.volume=176&rft.issue=3&rft.spage=e14394&rft.epage=n/a&rft.pages=e14394-n/a&rft.issn=0031-9317&rft.eissn=1399-3054&rft_id=info:doi/10.1111/ppl.14394&rft_dat=%3Cproquest_cross%3E3070803164%3C/proquest_cross%3E%3Cgrp_id%3Ecdi_FETCH-LOGICAL-c2434-8dde19ce408f7697911a59904a625dfd196e3204bb1ee369df353521155966493%3C/grp_id%3E%3Coa%3E%3C/oa%3E%3Curl%3E%3C/url%3E&rft_id=info:oai/&rft_pqid=3071990041&rft_id=info:pmid/38894535&rfr_iscdi=true |