Selective Activity Against Amastigote Forms of Trypanosoma cruzi and Leishmania infantum of Diastereomeric Dicentrine N‐oxides

• Published in: Chemistry & biodiversity 2024-09, Vol.21 (9), p.e202401247-n/a
• Main Authors: Tristão, Daniela C., Barbosa, Henrique, Castro Levatti, Erica V., Andrade, Beatriz A., Romanelli, Maiara M., Antar, Guilherme M., Tempone, Andre G., Lago, João Henrique G.
• Format: Article
• Language: English
• Subjects: Amastigotes; Animals; Antileishmanial; Antiparasitic agents; Antiprotozoal Agents - chemical synthesis; Antiprotozoal Agents - chemistry; Antiprotozoal Agents - pharmacology; Antitrypanosomal; Aporphine alkaloids; Bioactive compounds; Biocompatibility; Biological activity; Cell Line; Cytotoxicity; Diastereoisomers; Dose-Response Relationship, Drug; Leishmania infantum; Leishmania infantum - drug effects; Mice; Miltefosine; Molecular Structure; N-oxide; Oxidation; Parasites; Parasitic Sensitivity Tests; Protozoa; Stereoisomerism; Structure-Activity Relationship; Trypanosoma cruzi; Trypanosoma cruzi - drug effects
• ISSN: 1612-1872; 1612-1880; 1612-1880
• DOI: 10.1002/cbdv.202401247

As part of our continuous research for the discovery of bioactive compounds against Trypanosoma cruzi and Leishmania infantum, the alkaloid (6aS)‐dicentrine (1) was oxidized to afford (6aS,6S)‐ (2) and (6aS,6R)‐ (3) dicentrine‐N‐oxides. Evaluation of the cytotoxicity against NCTC cells indicated that 2 and 3 are non‐toxic (CC50>200 μM) whereas 1 demonstrated CC50 of 52.0 μM. Concerning T. cruzi activity against amastigotes, derivatives 2 and 3 exhibited EC50 values of 9.9 μM (SI>20.2) and 27.5 μM (SI>7.3), respectively, but 1 is inactive (EC50>100 μM). Otherwise, when tested against L. infantum amastigotes, 1 and 3 exhibited EC50 values of 10.3 μM (SI=5.0) and 12.7 μM (SI>15.7), respectively, being 2 inactive (EC50>100 μM). Comparing the effects of positive controls benznidazol (EC50=6.5 μM and SI>30.7) and miltefosine (EC50=10.2 μM and SI=15.2), it was observed a selective antiparasitic activity to diastereomers 2 and 3 against T. cruzi and L. infantum. Considering stereochemical aspects, it was suggested that the configuration of the new stereocenter formed after oxidation of 1 played an important role in the bioactivity against amastigotes of both tested parasites.