Promotion and Mechanism of Acupotomy on Chondrocyte Autophagy in Knee Osteoarthritis Rabbits

Objective To explore the effect of acupotomy intervention on autophagy of chondrocytes in rabbits with knee osteoarthritis (KOA), and to determine the possible mechanisms of acupotomy to alleviate cartilage degeneration. Methods The modified Videman method was used to construct a KOA rabbit model. A...

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Published in:Chinese journal of integrative medicine 2024-09, Vol.30 (9), p.809-817
Main Authors: Lu, Man, Meng, De-hong, She, Ze-yu, Wu, Xian, Xia, Shuai, Yang, Kai-ning, Liu, Cun-bin, Li, Tao, Yang, Yong-hui
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container_title Chinese journal of integrative medicine
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Meng, De-hong
She, Ze-yu
Wu, Xian
Xia, Shuai
Yang, Kai-ning
Liu, Cun-bin
Li, Tao
Yang, Yong-hui
description Objective To explore the effect of acupotomy intervention on autophagy of chondrocytes in rabbits with knee osteoarthritis (KOA), and to determine the possible mechanisms of acupotomy to alleviate cartilage degeneration. Methods The modified Videman method was used to construct a KOA rabbit model. After modeling, 40 rabbits were randomly divided into 4 groups by a random number table: control; KOA (model); KOA + acupotomy (acupotomy), and KOA + sham acupotomy (sham), 10 in each group. After a 3-week treatment course, the knee joint activity was determined by the modified Lequesne MG index. Hematoxylin-eosin staining staining was used to examine the morphological changes of chondrocytes. Autophagy of chondrocytes was observed by transmission electron microscopy. The surface morphology of cartilage tissue was observed by scanning electron microscope. The mRNA and protein levels of AMP kinase/mammalian target of rapamycin/Unc-51 (AMPK/mTOR/ULK1) signal pathway key proteins, autophagy-related factor Beclin-1 and microtubule-associated protein 1A/1B light chain 3 (LC3) in rabbit knee cartilage were assessed by real-time fluorescence quantitative polymerase chain reaction and Western blot, respectively. Results The modified Lequesne MG score of acupotomy group was significantly lower than that of model group ( P
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Methods The modified Videman method was used to construct a KOA rabbit model. After modeling, 40 rabbits were randomly divided into 4 groups by a random number table: control; KOA (model); KOA + acupotomy (acupotomy), and KOA + sham acupotomy (sham), 10 in each group. After a 3-week treatment course, the knee joint activity was determined by the modified Lequesne MG index. Hematoxylin-eosin staining staining was used to examine the morphological changes of chondrocytes. Autophagy of chondrocytes was observed by transmission electron microscopy. The surface morphology of cartilage tissue was observed by scanning electron microscope. The mRNA and protein levels of AMP kinase/mammalian target of rapamycin/Unc-51 (AMPK/mTOR/ULK1) signal pathway key proteins, autophagy-related factor Beclin-1 and microtubule-associated protein 1A/1B light chain 3 (LC3) in rabbit knee cartilage were assessed by real-time fluorescence quantitative polymerase chain reaction and Western blot, respectively. Results The modified Lequesne MG score of acupotomy group was significantly lower than that of model group ( P &lt;0.05). Pathological results showed that chondrocyte autophagy decreased and cartilage surface was rough in the model group, which recovered after acupotomy treatment. The mRNA expressions of AMPK, ULK1, Beclin-1 and the protein levels of p-AMPK, p-ULK1, Beclin-1, and LC3 II/LC3 I were decreased in the model group, while the mRNA and protein expressions of mTOR were increased ( P &lt;0.01). However, acupotomy treatment reversed these abnormal changes ( P &lt;0.05). Conclusions Acupotomy could effectively up-regulate the expressions of AMPK, ULK1 and Beclin1, reduce the expression of mTOR, promote autophagy, and alleviate joint degeneration. Acupotomy is a promising complementary and alternative therapy for KOA.</description><identifier>ISSN: 1672-0415</identifier><identifier>ISSN: 1993-0402</identifier><identifier>EISSN: 1993-0402</identifier><identifier>DOI: 10.1007/s11655-024-3759-8</identifier><identifier>PMID: 38900226</identifier><language>eng</language><publisher>Singapore: Springer Nature Singapore</publisher><subject>Medicine ; Medicine &amp; Public Health ; Original Article</subject><ispartof>Chinese journal of integrative medicine, 2024-09, Vol.30 (9), p.809-817</ispartof><rights>The Chinese Journal of Integrated Traditional and Western Medicine Press and Springer-Verlag GmbH Germany, part of Springer Nature 2024</rights><rights>2024. The Chinese Journal of Integrated Traditional and Western Medicine Press and Springer-Verlag GmbH Germany, part of Springer Nature.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><cites>FETCH-LOGICAL-c226t-a98bd9e59f3078bc8a0bbb098fba18920f789ef769f6e00a859a6ea147f11a823</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27924,27925</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/38900226$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Lu, Man</creatorcontrib><creatorcontrib>Meng, De-hong</creatorcontrib><creatorcontrib>She, Ze-yu</creatorcontrib><creatorcontrib>Wu, Xian</creatorcontrib><creatorcontrib>Xia, Shuai</creatorcontrib><creatorcontrib>Yang, Kai-ning</creatorcontrib><creatorcontrib>Liu, Cun-bin</creatorcontrib><creatorcontrib>Li, Tao</creatorcontrib><creatorcontrib>Yang, Yong-hui</creatorcontrib><title>Promotion and Mechanism of Acupotomy on Chondrocyte Autophagy in Knee Osteoarthritis Rabbits</title><title>Chinese journal of integrative medicine</title><addtitle>Chin. J. Integr. Med</addtitle><addtitle>Chin J Integr Med</addtitle><description>Objective To explore the effect of acupotomy intervention on autophagy of chondrocytes in rabbits with knee osteoarthritis (KOA), and to determine the possible mechanisms of acupotomy to alleviate cartilage degeneration. Methods The modified Videman method was used to construct a KOA rabbit model. After modeling, 40 rabbits were randomly divided into 4 groups by a random number table: control; KOA (model); KOA + acupotomy (acupotomy), and KOA + sham acupotomy (sham), 10 in each group. After a 3-week treatment course, the knee joint activity was determined by the modified Lequesne MG index. Hematoxylin-eosin staining staining was used to examine the morphological changes of chondrocytes. Autophagy of chondrocytes was observed by transmission electron microscopy. The surface morphology of cartilage tissue was observed by scanning electron microscope. The mRNA and protein levels of AMP kinase/mammalian target of rapamycin/Unc-51 (AMPK/mTOR/ULK1) signal pathway key proteins, autophagy-related factor Beclin-1 and microtubule-associated protein 1A/1B light chain 3 (LC3) in rabbit knee cartilage were assessed by real-time fluorescence quantitative polymerase chain reaction and Western blot, respectively. Results The modified Lequesne MG score of acupotomy group was significantly lower than that of model group ( P &lt;0.05). Pathological results showed that chondrocyte autophagy decreased and cartilage surface was rough in the model group, which recovered after acupotomy treatment. The mRNA expressions of AMPK, ULK1, Beclin-1 and the protein levels of p-AMPK, p-ULK1, Beclin-1, and LC3 II/LC3 I were decreased in the model group, while the mRNA and protein expressions of mTOR were increased ( P &lt;0.01). However, acupotomy treatment reversed these abnormal changes ( P &lt;0.05). Conclusions Acupotomy could effectively up-regulate the expressions of AMPK, ULK1 and Beclin1, reduce the expression of mTOR, promote autophagy, and alleviate joint degeneration. Acupotomy is a promising complementary and alternative therapy for KOA.</description><subject>Medicine</subject><subject>Medicine &amp; Public Health</subject><subject>Original Article</subject><issn>1672-0415</issn><issn>1993-0402</issn><issn>1993-0402</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2024</creationdate><recordtype>article</recordtype><recordid>eNp9kEtPAyEUhYnR-Kj-ADeGpZvRC1MGWDaNr6ipMbozITCFdkwHKjCL_ntpqi5d3ZPcc07u_RA6J3BFAPh1IqRhrAI6rmrOZCX20DGRsq5gDHS_6IbTogk7QicpfQIw3gA7REe1kACUNsfo4yWGPuQueKz9HD_bdql9l3ocHJ60wzrk0G9w2U6Xwc9jaDfZ4smQw3qpFxvcefzorcWzlG3QMS9jl7uEX7UxXU6n6MDpVbJnP3OE3m9v3qb31dPs7mE6earackOutBRmLi2TrgYuTCs0GGNACmc0EZKC40JaxxvpGgugBZO6sZqMuSNEC1qP0OWudx3D12BTVn2XWrtaaW_DkFSpBUE5YVsr2VnbGFKK1ql17HodN4qA2kJVO6iqQFVbqEqUzMVP_WB6O_9L_FIsBrozpLLyCxvVZxiiLy__0_oNcLyC6Q</recordid><startdate>20240901</startdate><enddate>20240901</enddate><creator>Lu, Man</creator><creator>Meng, De-hong</creator><creator>She, Ze-yu</creator><creator>Wu, Xian</creator><creator>Xia, Shuai</creator><creator>Yang, Kai-ning</creator><creator>Liu, Cun-bin</creator><creator>Li, Tao</creator><creator>Yang, Yong-hui</creator><general>Springer Nature Singapore</general><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20240901</creationdate><title>Promotion and Mechanism of Acupotomy on Chondrocyte Autophagy in Knee Osteoarthritis Rabbits</title><author>Lu, Man ; Meng, De-hong ; She, Ze-yu ; Wu, Xian ; Xia, Shuai ; Yang, Kai-ning ; Liu, Cun-bin ; Li, Tao ; Yang, Yong-hui</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c226t-a98bd9e59f3078bc8a0bbb098fba18920f789ef769f6e00a859a6ea147f11a823</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2024</creationdate><topic>Medicine</topic><topic>Medicine &amp; Public Health</topic><topic>Original Article</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Lu, Man</creatorcontrib><creatorcontrib>Meng, De-hong</creatorcontrib><creatorcontrib>She, Ze-yu</creatorcontrib><creatorcontrib>Wu, Xian</creatorcontrib><creatorcontrib>Xia, Shuai</creatorcontrib><creatorcontrib>Yang, Kai-ning</creatorcontrib><creatorcontrib>Liu, Cun-bin</creatorcontrib><creatorcontrib>Li, Tao</creatorcontrib><creatorcontrib>Yang, Yong-hui</creatorcontrib><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Chinese journal of integrative medicine</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Lu, Man</au><au>Meng, De-hong</au><au>She, Ze-yu</au><au>Wu, Xian</au><au>Xia, Shuai</au><au>Yang, Kai-ning</au><au>Liu, Cun-bin</au><au>Li, Tao</au><au>Yang, Yong-hui</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Promotion and Mechanism of Acupotomy on Chondrocyte Autophagy in Knee Osteoarthritis Rabbits</atitle><jtitle>Chinese journal of integrative medicine</jtitle><stitle>Chin. J. Integr. Med</stitle><addtitle>Chin J Integr Med</addtitle><date>2024-09-01</date><risdate>2024</risdate><volume>30</volume><issue>9</issue><spage>809</spage><epage>817</epage><pages>809-817</pages><issn>1672-0415</issn><issn>1993-0402</issn><eissn>1993-0402</eissn><abstract>Objective To explore the effect of acupotomy intervention on autophagy of chondrocytes in rabbits with knee osteoarthritis (KOA), and to determine the possible mechanisms of acupotomy to alleviate cartilage degeneration. Methods The modified Videman method was used to construct a KOA rabbit model. After modeling, 40 rabbits were randomly divided into 4 groups by a random number table: control; KOA (model); KOA + acupotomy (acupotomy), and KOA + sham acupotomy (sham), 10 in each group. After a 3-week treatment course, the knee joint activity was determined by the modified Lequesne MG index. Hematoxylin-eosin staining staining was used to examine the morphological changes of chondrocytes. Autophagy of chondrocytes was observed by transmission electron microscopy. The surface morphology of cartilage tissue was observed by scanning electron microscope. The mRNA and protein levels of AMP kinase/mammalian target of rapamycin/Unc-51 (AMPK/mTOR/ULK1) signal pathway key proteins, autophagy-related factor Beclin-1 and microtubule-associated protein 1A/1B light chain 3 (LC3) in rabbit knee cartilage were assessed by real-time fluorescence quantitative polymerase chain reaction and Western blot, respectively. Results The modified Lequesne MG score of acupotomy group was significantly lower than that of model group ( P &lt;0.05). Pathological results showed that chondrocyte autophagy decreased and cartilage surface was rough in the model group, which recovered after acupotomy treatment. The mRNA expressions of AMPK, ULK1, Beclin-1 and the protein levels of p-AMPK, p-ULK1, Beclin-1, and LC3 II/LC3 I were decreased in the model group, while the mRNA and protein expressions of mTOR were increased ( P &lt;0.01). However, acupotomy treatment reversed these abnormal changes ( P &lt;0.05). Conclusions Acupotomy could effectively up-regulate the expressions of AMPK, ULK1 and Beclin1, reduce the expression of mTOR, promote autophagy, and alleviate joint degeneration. Acupotomy is a promising complementary and alternative therapy for KOA.</abstract><cop>Singapore</cop><pub>Springer Nature Singapore</pub><pmid>38900226</pmid><doi>10.1007/s11655-024-3759-8</doi><tpages>9</tpages></addata></record>
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Original Article
title Promotion and Mechanism of Acupotomy on Chondrocyte Autophagy in Knee Osteoarthritis Rabbits
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