The CRISPR-Cas system in molecular diagnostics

[Display omitted] •We reviewed and summarized existing studies on the classification and mechanisms of the CRISPR-Cas system.•We list representative detection tools based on different CRISPR-Cas systems and discuss the advantages and disadvantages.•We discuss more possibilities for the CRISPR-Cas sy...

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Bibliographic Details
Published in:Clinica chimica acta 2024-07, Vol.561, p.119820, Article 119820
Main Authors: Song, Rao, Chen, Zhongyi, Xiao, Hongtao, Wang, Haojun
Format: Article
Language:English
Subjects:
Biosensor
Cas12
Cas13
Cas9
COVID-19
CRISPR
Detection
Molecular diagnosis
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Summary:[Display omitted] •We reviewed and summarized existing studies on the classification and mechanisms of the CRISPR-Cas system.•We list representative detection tools based on different CRISPR-Cas systems and discuss the advantages and disadvantages.•We discuss more possibilities for the CRISPR-Cas system in the field of detection. Robust, sensitive, and rapid molecular detection tools are essential prerequisites for disease diagnosis and epidemiological control. However, the current mainstream tests necessitate expensive equipment and specialized operators, impeding the advancement of molecular diagnostics. The CRISPR-Cas system is an integral component of the bacterial adaptive immune system, wherein Cas proteins recognize PAM sequences by binding to CRISPR RNA, subsequently triggering DNA or RNA cleavage. The discovery of the CRISPR-Cas system has invigorated molecular diagnostics. With further in-depth research on this system, its application in molecular diagnosis is flourishing. In this review, we provide a comprehensive overview of recent research progress on the CRISPR-Cas system, specifically focusing on its application in molecular diagnosis.
ISSN:0009-8981
1873-3492
1873-3492
DOI:10.1016/j.cca.2024.119820