A histopathological analysis of extrapulmonary lesions in fatal coronavirus disease (COVID-19)

• Published in: Pathology, research and practice research and practice, 2024-08, Vol.260, p.155373, Article 155373
• Main Authors: Gheban-Roşca, Ioana-Andreea, Gheban, Bogdan-Alexandru, Pop, Bogdan, Mironescu, Daniela-Cristina, Siserman, Vasile Costel, Jianu, Elena Mihaela, Drugan, Tudor, Bolboacă, Sorana D.
• Format: Article
• Language: English
• Subjects: Autopsy; COVID-19; Diffuse alveolar damage; Histopathology; Multi-organ involvement; SARS-CoV-2
• ISSN: 0344-0338; 1618-0631; 1618-0631
• DOI: 10.1016/j.prp.2024.155373

The coronavirus disease 2019 (COVID-19) pandemic, caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), presents diverse clinical manifestations and multi-organ involvement. This study aimed to evaluate the extra-pulmonary histopathological patterns underpinning COVID-19-induced lesions in cardiac, hepatic, renal, brainstem, and splenic tissues. The research involved conventional forensic autopsies conducted between April 2020 and April 2021 on individuals with confirmed SARS-CoV-2 infection in Cluj-Napoca, Romania. Tissues were processed and stained for histological examination. Differences in patients with and without diffuse alveolar damage (DAD) were evaluated. In our study of 79 COVID-19 autopsies conducted on unvaccinated patients besides lung involvement, the patients had histological changes in at least two out of five (brain, heart, liver, kidney, and spleen) organs. Notable findings include hepatitis observed in 46.8 % of cases, 21.5 % with lobular hepatitis, and 41.8 % with liver steatosis. Additionally, 69.6 % exhibited acute tubular necrosis, and 55.7 % had varying degrees of splenic lymphocyte depletion. Almost 41 % of cases had pericardial effusion, 36.7 % myocarditis, 24.1 % myocardial infarction, and 12.7 % of cases had encephalitis. Acute tubular necrosis (78.6 %) was the most frequent histopathological finding observed in patients with DAD. Myocarditis was described in 45.9 % of the patients without DAD. The autopsy findings in our cohort of COVID-19 victims align with international scientific literature. Distinguishing viral-induced myocarditis, encephalitis, hepatitis, or systemic inflammatory syndrome remains challenging. Post-mortem analysis identified lesions associated with SARS-CoV-2 in multiple organs, highlighting the systemic nature of the virus and emphasizing the need for continued research into organ-specific damage and long-term sequelae of COVID-19. •Multi-organ involvement in COVID-19.•Systemic inflammatory lesions: encephalitis, myocarditis, and hepatitis.•Acute tubular necrosis was observed in over half of COVID-19 autopsies.•Different grades of splenic lymphocytic depletion were observed.•The mechanisms of COVID-19-associated organ damage are complex.