A rapid near-infrared turn on fluorescence probe for the detection of sulfite in food and its application in biological imaging

• Published in: Talanta (Oxford) 2024-10, Vol.278, p.126445, Article 126445
• Main Authors: Li, Shufei, Hong, Jiaxin, Xia, Xinyu, Duan, Luying, Yang, Wuying, Xiong, Jianhua, Yin, Xiaoli, Hong, Yanping
• Format: Article
• Language: English
• Subjects: Animals; Bioimaging; Fluorescent Dyes - chemistry; Food Analysis - methods; Food Contamination - analysis; HeLa Cells; Humans; Limit of Detection; Near-infrared fluorescent probe; Onions - chemistry; Optical Imaging - methods; Real food samples; Spectrometry, Fluorescence - methods; Sulfite; Sulfites - analysis; Sulfites - chemistry; Wine - analysis; Zebrafish
• ISSN: 0039-9140; 1873-3573; 1873-3573
• DOI: 10.1016/j.talanta.2024.126445

A near-infrared fluorescent “turn on” probe DTMI featuring simple skeleton was constructed easily. It undergoes a structure transformation from an A−π−A to a D−π−A framework towards SO32−. Besides, DTMI is capable of distinctive sensing sulfite with a fast response and a significant Stokes shift as well as with high sensitivity, excellent selectivity, long-term stability of fluorescence signals, and good anti-interference ability. The detection limit (LOD) of DTMI for sulfite within the linear concentration range of 0.5–10 μM is 27.39 nM. More importantly, DTMI has been favorably utilized for detecting sulfite in food samples such as red wine and vermicelli. Based on its low biotoxicity, DTMI has been successfully applied in imaging experiments involving HeLa cells, onion inner epidermal cells, and zebrafish. Therefore, the results show that the presented probe possesses potential sensing activity towards sulfite in complex biological system and food samples. [Display omitted] •Turn on fluorescent emission derived from structural alteration from A−π−A to D−π−A.•Rapid and accurate detection of SO2 with high sensitivity and selectivity.•Stable near-infrared fluorescence signal with a nanomolar LOD.•Sniffing sulfite within food samples and living cells.