Biomimetic nanoplatforms constructed from dialkylaminostyryl hetarene dyes and phospholipids exhibiting selective fluorescent response to specific proteins

• Published in: Colloids and surfaces, B, Biointerfaces B, Biointerfaces, 2024-09, Vol.241, p.114046, Article 114046
• Main Authors: Akhmadeev, Bulat, Retyunskaya, Olga, Islamova, Liliya, Fazleeva, Guzyal, Kalinin, Alexey, Katsyuba, Sergey, Elistratova, Julia, Sinyashin, Oleg, Mustafina, Asiya
• Format: Article
• Language: English
• Subjects: Luminescent sensor; Phospholipid bilayers; Protein interaction
• ISSN: 0927-7765; 1873-4367; 1873-4367
• DOI: 10.1016/j.colsurfb.2024.114046

The present work explores the specificity of supramolecular assemblies comprising dialkylaminostyrylhetarene dye molecules incorporated into phosphatidylcholine (PC) or phosphatidylserine (PS) aggregates. In PS-based assemblies, the dyes demonstrate a concentration-dependent fluorescent response, distinguishing anionic proteins such as bovine serum albumin (BSA) and pepsin from lysozyme (LYZ) in aqueous solutions. Conversely, no significant response is observed when the dyes are incorporated into the well-organized bilayers of neutral PC. The fluorescent response arises from the binding of dyes to proteins, leading to the detachment of dye molecules from the assemblies, rather than from the binding of proteins to the assemblies, although the latter process is facilitated by electrostatic attraction. Thus, both the poor ordering of PS molecules and the interfacial arrangement of the dyes are prerequisites for the fluorescent response of dye-PS aggregates. The structure of the dyes significantly impacts the spectral features of dye-PS and dye-protein assemblies. An optimal dye structure has been identified for the recognition of BSA, with a limit of detection (LOD) of 10.8 nM. •Construction of nanosensor by self-assembly of fluorescent dyes and phospholipids.•Selective response of the nanosensor to anionic proteins due to the dye-protein binding.•Sensitivity of the nanosensor is ensured by the interfacial arrangement of dyes.•Supramolecular disordering of phospholipids supports interfacial localization of dyes.•Determination of bovine serum albumin with a lower limit of detection of 10.8 nM.