Morphological and functional evolution of gametophytes in epilithic Hymenasplenium murakami-hatanakae (Aspleniaceae): The fifth family capable of producing the independent gametophytes

• Published in: Journal of plant research 2024-09, Vol.137 (5), p.815-828
• Main Authors: Yoneoka, Katsuhiro, Fujiwara, Tao, Kataoka, Toshifumi, Hori, Kiyotaka, Ebihara, Atsushi, Murakami, Noriaki
• Format: Article
• Language: English
• Subjects: Aspleniaceae; Biological Evolution; Biomedical and Life Sciences; Chloroplast DNA; Chloroplasts; DNA, Chloroplast - genetics; Environmental factors; Ferns; Ferns - genetics; Ferns - physiology; Gametophytes; Germ Cells, Plant - physiology; Islands; Japan; Life Sciences; Morphology; Phylogeny; Plant Biochemistry; Plant Ecology; Plant Physiology; Plant Sciences; Populations; Regular Paper – Taxonomy/Phylogenetics/Evolutionary Biology; Reproduction; Spores; Sporophytes; Vegetative reproduction
• ISSN: 0918-9440; 1618-0860; 1618-0860
• DOI: 10.1007/s10265-024-01553-0

The fern independent gametophytes that can maintain populations by vegetative reproduction without conspecific sporophytes have been considered an unusual phenomenon found in some epiphytic or epilithic species of Hymenophyllaceae, Pteridaceae, Lomariopsidaceae, and Polypodiaceae. By chance, the discovery of mysterious strap-like gametophytes on Izu-Oshima Island, Japan, has led to the hypothesis that Hymenasplenium murakami-hatanakae , a fern species belonging to Aspleniaceae, can also form independent gametophytes. Our investigation revealed gametophyte populations of H. murakami-hatanakae on three islands in the Izu Islands. Based on chloroplast DNA analysis of the gametophyte and sporophyte populations, the gametophytes were found to be maintained by vegetative reproduction without a new supply of spores from sporophytes. A comparison of the surrounding vegetation at the collection sites showed that environmental factors such as light and humidity may influence the maintenance of gametophyte populations. These results clearly show that H. murakami-hatanakae is one of the ferns capable of forming independent gametophytes. This is the first report of independent gametophytes from the suborder Aspleniineae (eupolypod II). The discovery of the independent gametophyte within a phylogenetic lineage previously thought not to form independent gametophytes will provide important insights into the morphological and functional evolution of gametophytes in ferns.