A fungal‐binding agglutinin in the skin slime of Japanese flounder (Paralichthys olivaceus) is glyceraldehyde 3‐phosphate dehydrogenase

Agglutination of pathogenic microorganisms on the body surface is a significant phenomenon for the prevention of infection. In the present study, we show that an extract of the skin mucus from Japanese flounder (Paralichthys olivaceus) has agglutination activity against the yeast Saccharomyces cerev...

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Bibliographic Details
Published in:Microbiology and immunology 2024-09, Vol.68 (9), p.331-338
Main Authors: Tsutsui, Shigeyuki, Terashima, Mizuki, Nakamura, Osamu
Format: Article
Language:English
Subjects:
Affinity chromatography
Agglutination
agglutinin
fungi
GAPDH
Glyceraldehyde
Glyceraldehyde-3-phosphate dehydrogenase
Glycolysis
innate immunity
Japanese flounder
Liquid chromatography
Mass spectroscopy
Mucosal immunity
Mucus
Nucleotide sequence
Paralichthys olivaceus
Skin
skin mucus
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Summary:Agglutination of pathogenic microorganisms on the body surface is a significant phenomenon for the prevention of infection. In the present study, we show that an extract of the skin mucus from Japanese flounder (Paralichthys olivaceus) has agglutination activity against the yeast Saccharomyces cerevisiae. We purified this yeast‐binding protein, which consists of an approximately 35‐kDa homodimer, using affinity chromatography with yeast as a ligand. Multiple internal amino acid sequences of the protein, as determined using liquid chromatography with quadrupole time‐of‐flight tandem mass spectrometry, mapped to flounder glyceraldehyde 3‐phosphate dehydrogenase (GAPDH). An anti‐GAPDH antibody inhibited the yeast agglutination activity in the skin mucus extract and stained agglutinated yeast, indicating that flounder GAPDH could agglutinate yeast. The current study suggests that GAPDH, a well‐known protein as the sixth enzyme in the glycolytic pathway, is a significant player in mucosal immunity in teleosts.
ISSN:0385-5600
1348-0421
1348-0421
DOI:10.1111/1348-0421.13163