Effects of Active Chronic Cigarette-Smoke Exposure on Circulating Fibrocytes

Purpose This study aimed to evaluate the hypothesis that active smoking impacts upon mediators and abundance of circulating fibrocyte cells in smoking-related disease characterised by fibrosis. Methods Flow cytometry and enzyme-linked immunosorbent assays were used to investigate blood from five pat...

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Bibliographic Details
Published in:Lung 2024-08, Vol.202 (4), p.431-440
Main Authors: Khan, Faheem, Judge, Eoin P., Das, Jeeban P., White, Daniel, Ingram, Carolyn, Keane, Michael P., Butler, Marcus W.
Format: Article
Language:English
Subjects:
Adult
Aged
Blood circulation
Case-Control Studies
Chemokine CCL2 - blood
Chemokine CXCL12 - blood
Chemokines
Chemokines, CC - blood
Chronic obstructive pulmonary disease
Cigarette Smoking - adverse effects
Cigarette Smoking - blood
CXCL12 protein
Enzyme-Linked Immunosorbent Assay
Female
Fibrosis
Flow Cytometry
Humans
Idiopathic Pulmonary Fibrosis - blood
Idiopathic Pulmonary Fibrosis - pathology
Immunoassays
Lung diseases
Male
Medicine
Medicine & Public Health
Middle Aged
Monocyte chemoattractant protein 1
Non-Smokers - statistics & numerical data
Pneumology/Respiratory System
Population studies
Pulmonary Disease, Chronic Obstructive - blood
Pulmonary Disease, Chronic Obstructive - pathology
Serum levels
Smokers
Smoking
Smoking - adverse effects
Smoking - blood
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Summary:Purpose This study aimed to evaluate the hypothesis that active smoking impacts upon mediators and abundance of circulating fibrocyte cells in smoking-related disease characterised by fibrosis. Methods Flow cytometry and enzyme-linked immunosorbent assays were used to investigate blood from five patient groups: healthy never-smokers, healthy current smokers, stable chronic obstructive pulmonary disease (COPD) active smokers, idiopathic pulmonary fibrosis (IPF) never-smokers, and IPF active smokers. Results A significant inverse dose–response relationship was observed in healthy smokers among cumulative smoking burden (pack-years) and fibrocyte abundance ( p  = 0.006, r  = −0.86). Among serum profibrotic fibrocyte chemokines measured, CCL18 rose significantly alongside fibrocyte numbers in all five subject groups, while having an inverse dose–response relationship with pack-year burden in healthy smokers ( p  = 0.003, r  = −0.89). In IPF, CCL2 rose in direct proportion to fibrocyte abundance irrespective of smoking status but had lower serum levels in those currently smoking ( p  =  
ISSN:0341-2040
1432-1750
1432-1750
DOI:10.1007/s00408-024-00720-3