An F-box Kelch repeat protein, PmFBK2, from Persicaria minor interacts with GID1b to modulate gibberellin signalling

• Published in: Journal of plant physiology 2024-09, Vol.300, p.154299, Article 154299
• Main Authors: Abd-Hamid, Nur-Athirah, Ismail, Ismanizan
• Format: Article
• Language: English
• Subjects: Arabidopsis; Arabidopsis - genetics; Arabidopsis - metabolism; F-box proteins; F-Box Proteins - genetics; F-Box Proteins - metabolism; family; gene expression; Gene Expression Regulation, Plant; genes; Gibberellin signalling; gibberellins; Gibberellins - metabolism; kingdom; mutants; Persicaria minor; plant growth; Plant growth and development; Plant Growth Regulators - metabolism; plant height; Plant Proteins - genetics; Plant Proteins - metabolism; precipitin tests; SCF complex; seed weight; Signal Transduction; Skp1-dependent FBP; Two-Hybrid System Techniques; Ubiquitin – 26S proteasome system; ubiquitin-protein ligase
• ISSN: 0176-1617; 1618-1328; 1618-1328
• DOI: 10.1016/j.jplph.2024.154299

The F-box protein (FBP) family plays diverse functions in the plant kingdom, with the function of many members still unrevealed. In this study, a specific FBP called PmFBK2, containing Kelch repeats from Persicaria minor, was functionally investigated. Employing the yeast two-hybrid (Y2H) assay, PmFBK2 was found to interact with Skp1-like proteins from P. minor, suggesting its potential to form an E3 ubiquitin ligase, known as the SCF complex. Y2H and co-immunoprecipitation tests revealed that PmFBK2 interacts with full-length PmGID1b. The interaction marks the first documented binding between these two protein types, which have never been reported in other plants before, and they exhibited a negative effect on gibberellin (GA) signal transduction. The overexpression of PmFBK2 in the kmd3 mutant, a homolog from Arabidopsis, demonstrated the ability of PmFBK2 to restore the function of the mutated KMD3 gene. The function restoration was supported by morphophysiological and gene expression analyses, which exhibited patterns similar to the wild type (WT) compared to the kmd3 mutant. Interestingly, the overexpression of PmFBK2 or PmGID1b in Arabidopsis had opposite effects on rosette diameter, seed weight, and plant height. This study provides new insights into the complex GA signalling. It highlights the crucial roles of the interaction between FBP and the GA receptor (GID1b) in regulating GA responses. These findings have implications for developing strategies to enhance plant growth and yield by modulating GA signalling in crops.