Radicle growth regulation of root parasitic plants by auxin-related compounds

• Published in: Plant and cell physiology 2024-10, Vol.65 (9), p.1377-1387
• Main Authors: Tsuzuki, Kei, Suzuki, Taiki, Kuruma, Michio, Nishiyama, Kotaro, Hayashi, Ken-Ichiro, Hagihara, Shinya, Seto, Yoshiya
• Format: Article
• Language: English
• ISSN: 0032-0781; 1471-9053; 1471-9053
• DOI: 10.1093/pcp/pcae071

Root parasitic plants in the Orobancheceae, such as Striga and Orobanche, cause significant damage to crop production. The germination step of these root parasitic plants is induced by host-root-derived strigolactones (SLs). After germination, the radicles elongate toward the host and invade the host root. We have previously discovered that a simple amino acid, tryptophan (Trp), as well as its metabolite, the plant hormone indole-3-acetic acid (IAA), can inhibit radicle elongation of Orobanche minor. These results suggest that auxin plays a crucial role in the radicle elongation step in root parasitic plants. In this report, we used various auxin chemical probes to dissect the auxin function in the radicle growth of O. minor and Striga hermonthica. We found that synthetic auxins inhibited radicle elongation. In addition, auxin receptor antagonist, auxinole, rescued the inhibition of radicle growth by exogenous IAA. Moreover, a polar transport inhibitor of auxin, N-1-naphthylphthalamic acid (NPA), affected radicle bending. We also proved that exogenously applied Trp is converted into IAA in O. minor seeds, and auxinole partly rescued this radicle elongation. Our data demonstrate a pivotal role of auxin in radicle growth. Thus, manipulation of auxin function in root parasitic plants should offer a useful approach to combat these parasites.