Mechanisms of synthetic bacterial flora YJ-1 to enhance cucumber resistance under combined phthalate-disease stresses

• Published in: Journal of environmental management 2024-08, Vol.365, p.121564, Article 121564
• Main Authors: Wang, Lei, Li, Zhe, Li, MingZe, Chen, YuXin, Zhang, Ying, Bao, WenJing, Wang, XiaoDong, Qi, ZeWei, Zhang, WenQian, Tao, Yue
• Format: Article
• Language: English
• Subjects: Biodegradation, Environmental; Biotic-abiotic stresses; Cucumis sativus - microbiology; Degradation; Disease Resistance; Ecological stability; Fusarium; Phthalic Acids; Plant Diseases - microbiology; Resistant to wilt; Soil Microbiology; Stress, Physiological; Synthetic flora
• ISSN: 0301-4797; 1095-8630; 1095-8630
• DOI: 10.1016/j.jenvman.2024.121564

Biotic and abiotic stresses have emerged as major constraints to agricultural production, causing irreversible adverse impacts on agricultural production systems and thus posing a threat to food security. In this study, a new strain of Bacillus subtilis DNYB-S1 was isolated from soil contaminated with Fusarium wilt. It was found that artificially synthetic flora (YJ-1) [Enterobacter sp. DNB-S2 and Rhodococcus pyridinovorans DNHP-S2, DNYB-S1] could effectively mitigate both biotic (Fusarium wilt) and abiotic (phthalates) sources of stresses, with the inhibition rate of YJ-1 resistant to wilt being 71.25% and synergistic degradation of 500 mg/L PAEs was 91.23%. The adaptive difference of YJ-1 was 0.59 and the ecological niche overlap value was −0.05 as determined by Lotka-Volterra modeling. These results indicate that YJ-1 has good ecological stability. The major degradation intermediates included 2-ethylhexyl benzoate (EHBA), phthalic acid (PA), diisobutyl phthalate (DIBP), and butyl benzoate, suggesting that YJ-1 can provide a more efficient pathway for PAEs degradation. In addition, there was metabolic mutualism among the strains that will selectively utilize the provided carbon source (some metabolites of PAEs) for growth. The pot experiment showed that YJ-1 with cucumber reduced the incidence of cucumber wilt by 45.31%. YJ-1 could reduce the concentration of PAEs (DBP: DEHP = 1:1) in soil species from 30 mg/kg to 4.26 mg/kg within 35 d, with a degradation efficiency of 85.81%. Meanwhile, the concentration of PAEs in cucumber was reduced to 0.01 mg/kg, indicating that YJ-1 is directly involved in the degradation of soil PAEs and the enhancement of plant immunity. In conclusion, this study provides a new perspective for the development of customized microbiomes for phytoremediation under combined biotic-abiotic stresses in agricultural production processes. [Display omitted] •A new strain of Bacillus DNYB-S1 was isolated from Fusarium wilt soil.•YJ-1 provide a more efficient pathway for PAEs degradation.•YJ-1 has good ecological stability and potential for agricultural applications.•YJ-1 improved resistance of cucumber to Fusarium wilt and PAEs.