Characterization of the organellar genomes of Mazzaella laminarioides and Mazzaella membranacea (Gigartinaceae, Rhodophyta)

• Published in: Journal of phycology 2024-08, Vol.60 (4), p.797-805
• Main Authors: Sepúlveda‐Espinoza, Francisco, Cofré‐Serrano, Angela, Veloso‐Valeria, Tomás, Quesada‐Calderon, Suany, Guillemin, Marie‐Laure
• Format: Article
• Language: English
• Subjects: Chile; Chloroplasts; Divergence; divergence time; Economic importance; Gene order; Genes; Genome, Chloroplast; Genome, Mitochondrial; Genomes; Geographical distribution; Mazzaella; mitogenome; Nonsense mutation; Nucleotide sequence; Open reading frames; Phylogenetics; Phylogeny; plastome; Plastomes; pseudo genes; red algae; Rhodophyta - classification; Rhodophyta - genetics
• ISSN: 0022-3646; 1529-8817; 1529-8817
• DOI: 10.1111/jpy.13478

Mazzaella, a genus with no genomic resources available, has extensive distribution in the cold waters of the Pacific, where they represent ecologically and economically important species. In this study, we aimed to sequence, assemble, and annotate the complete mitochondrial and chloroplast genomes from two Mazzaella spp. and characterize the intraspecific variation among them. We report for the first time seven whole organellar genomes (mitochondria: OR915856, OR947465, OR947466, OR947467, OR947468, OR947469, OR947470; chloroplast: OR881974, OR909680, OR909681, OR909682, OR909683, OR909684, OR909685) obtained through high‐throughput sequencing for six M. laminarioides sampled from three Chilean regions and one M. membranacea. Sequenced Mazzaella mitogenomes have identical gene number, gene order, and genome structure. The same results were observed for assembled plastomes. A total of 52 genes were identified in mitogenomes, and a total of 235 genes were identified in plastomes. Although the M. membranacea plastome included a full‐length pbsA gene, in all M. laminarioides samples, the pbsA gene was split in three open reading frames (ORFs). Within M. laminarioides, we observed important plastome lineage‐specific variations, such as the pseudogenization of the two hypothetical protein‐coding genes, ycf23 and ycf45. Nonsense mutations in the ycf23 and ycf45 genes were only detected in the northern lineage. These results are consistent with phylogenetic reconstructions and divergence time estimation using concatenated coding sequences that not only support the monophyly of M. laminarioides but also underscore that the three M. laminarioides lineages are in an advanced stage of divergence. These new results open the question of the existence of still undisclosed species in M. laminarioides.