Phenotypic plasticity rather than ecotypic differentiation explains the broad realized niche of a Neotropical orchid species

• Published in: Plant biology (Stuttgart, Germany) Germany), 2024-10, Vol.26 (6), p.989-997
• Main Authors: Lima, T. M., Silva, S. F., Sánchez‐Vilas, J., Júnior, W. L. S., Mayer, J. L. S., Ribeiro, R. V., Pinheiro, F.
• Format: Article
• Language: English
• Subjects: Adaptation; Adaptation, Physiological; Climatic data; Common garden; Differentiation; Drought; Ecosystem; Ecotype; Genetic markers; Genetic structure; Genetic Variation; isolation by environment; Leaf area; Leaves; Microsatellite Repeats - genetics; Microsatellites; Moisture content; niche overlap; Niches; orchidaceae; Orchidaceae - genetics; Orchidaceae - physiology; Phenotype; Phenotypes; Phenotypic plasticity; Plant layout; Plant Leaves - genetics; Plant Leaves - physiology; Plant species; Plants (botany); Plastic properties; Plasticity; Population genetics; Population studies; Populations; Soil fertility; Soil stresses; Soil structure; Soil water; Stomata; Transplantation; Tropical Climate; Tropical plants; Water content
• ISSN: 1435-8603; 1438-8677; 1438-8677
• DOI: 10.1111/plb.13684

Local adaptation is common in plant species, and knowing whether a population is locally adapted has fundamental and applied relevance. However, local adaptation in tropical plants remains largely less studied, and covering this gap is not simple since reciprocal transplantation – the gold standard for detecting local adaptation – is not feasible for most species. Here, we combined genetic, climatic and phenotypic data to investigate ecotypic differentiation, an important aspect of local adaptation, in coastal and inland populations of the orchid Epidendrum fulgens Brongn., a long‐lived tropical plant for which reciprocal transplantation would not be feasible. We used nine microsatellite markers to estimate genetic divergence between inland and coastal populations. Moreover, occurrence data and climate data were used to test for differences in the realized niche of those populations. Finally, we assessed saturated water content, leaf specific area, height, and stomatal density in common garden and in situ to investigate the effects of ecotypic differentiation and plasticity on the phenotype. Coastal and inland groups' niches do not overlap, the former occupying a wetter and warmer area. However, this differentiation does not seem to be driven by ecotypic differentiation since there was no positive correlation between genetic structure and climate dissimilarity. Moreover, specific leaf area and leaf saturated water content, which are important phenotypic traits related to soil fertility and drought stress, were rather plastic. We conclude that ecotypic differentiation is absent, since phenotypic plasticity is an important mechanism explaining the niche broadness of this species. Niche analysis, population genetics and common garden experiments demonstrate that phenotypic plasticity, and not ecotypic differentiation explains the wide distribution of a Neotropical orchid species.