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Toward noncontact macroscopic imaging of multiple cancers using multi-spectral inelastic scattering detection

Here we introduce a Raman spectroscopy approach combining multi-spectral imaging and a new fluorescence background subtraction technique to image individual Raman peaks in less than 5 seconds over a square field-of-view of 1-centimeter sides with 350 micrometers resolution. First, human data is pres...

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Bibliographic Details
Published in:Journal of biophotonics 2024-09, Vol.17 (9), p.e202400087
Main Authors: David, Sandryne, Ksantini, Nassim, Dallaire, Frédérick, Ember, Katherine, Daoust, François, Sheehy, Guillaume, Hadjipanayis, Costas G, Petrecca, Kevin, Wilson, Brian C, Leblond, Frédéric
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Language:English
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Summary:Here we introduce a Raman spectroscopy approach combining multi-spectral imaging and a new fluorescence background subtraction technique to image individual Raman peaks in less than 5 seconds over a square field-of-view of 1-centimeter sides with 350 micrometers resolution. First, human data is presented supporting the feasibility of achieving cancer detection with high sensitivity and specificity - in brain, breast, lung, and ovarian/endometrium tissue - using no more than three biochemically interpretable biomarkers associated with the inelastic scattering signal from specific Raman peaks. Second, a proof-of-principle study in biological tissue is presented demonstrating the feasibility of detecting a single Raman band - here the CH /CH deformation bands from proteins and lipids - using a conventional multi-spectral imaging system in combination with the new background removal method. This study paves the way for the development of a new Raman imaging technique that is rapid, label-free, and wide field.
ISSN:1864-063X
1864-0648
1864-0648
DOI:10.1002/jbio.202400087