Phenolic Profile, Bioactivities and In Silico Analysis of the Trunk Bark of Acacia Cyanophylla Lindl

• Published in: Chemistry & biodiversity 2024-08, Vol.21 (8), p.e202401061-n/a
• Main Authors: Beyaoui, Ahlem, Kaplan, Muammer, Saidi, Ilyes, Jalouli, Maroua, Ceyhan Goren, Ahmet, Halim Harrath, Abdel, Ben Jannet, Hichem
• Format: Article
• Language: English
• Subjects: Acacia - chemistry; Acacia cyanophylla Lindl; alpha-Amylases - antagonists & inhibitors; alpha-Amylases - metabolism; Amylases; Antioxidants; Antioxidants - chemistry; Antioxidants - isolation & purification; Antioxidants - pharmacology; Bark; Bioactivities; Biphenyl Compounds - antagonists & inhibitors; Computer Simulation; Enzyme Inhibitors - chemistry; Enzyme Inhibitors - isolation & purification; Enzyme Inhibitors - pharmacology; Flavonoids; Flavonoids - chemistry; Flavonoids - isolation & purification; Flavonoids - pharmacology; In vitro methods and tests; Kojic acid; LC–HRMS; Molecular docking; Molecular Docking Simulation; Monophenol Monooxygenase - antagonists & inhibitors; Monophenol Monooxygenase - metabolism; Phenolic compounds; Phenols; Phenols - chemistry; Phenols - isolation & purification; Phenols - pharmacology; Picrates - antagonists & inhibitors; Plant Bark - chemistry; Plant extracts; Plant Extracts - chemistry; Plant Extracts - isolation & purification; Plant Extracts - pharmacology; Polyphenols; Reagents; Tyrosinase; α-Amylase
• ISSN: 1612-1872; 1612-1880; 1612-1880
• DOI: 10.1002/cbdv.202401061

In the current investigation, total phenolics and flavonoids of the methanolic extract obtained from the trunk bark of Acacia cyanophylla Lindl. were quantified by LC–HRMS technique. DPPH and ABTS reagents were employed to assay the antioxidant potential. The anti‐tyrosinase and anti‐α‐amylase potentials were also assayed. The findings revealed that thirteen polyphenolic compounds were detected in the methanolic extract with trans‐taxifolin (23.2 g/kg), as the major constituent. A. cyanophylla extract displayed a higher activity with DPPH test (IC50=10.14±1.00 μg/mL) than with ABTS (IC50=15.27±2.09 μg/mL). The same extract also exhibited interesting α‐amylase inhibitory action (IC50 value of 4.00±0.17 μg/mL). Moreover, methanolic trunk bark extract exerted strong anti‐tyrosinase capacity with an IC50 of 5.12±0.41 μg/mL in comparison to kojic acid (IC50=10.22±0.85 μg/mL) used as positive control. The antioxidant, anti‐tyrosinase and anti‐α‐amylase potentials of the methanolic extract of A. cyanophylla trunk bark were reinforced by in silico molecular docking analyses, which confirmed the results of the in vitro tests.