Chemico-nutritional characterization and anti-inflammatory potential of Chirabilva (Holoptelea integrifolia Roxb.) seed: alternate source of protein supplement and fatty acids

• Published in: Journal of food science and technology 2024-08, Vol.61 (8), p.1578-1588
• Main Authors: Srivastava, Nupur, Singh, Amrat Pal, Gupta, Princi, Gaur, Pooja, Bhatt, Divya, Khare, Puja, Bawankule, Dnyaneshwar U., Shanker, Karuna
• Format: Article
• Language: English
• Subjects: Carbohydrates; Chemistry; Chemistry and Materials Science; Chemistry/Food Science; Cytotoxicity; Dietary supplements; Electron transfer; Essential nutrients; Essential oils; Food Science; High protein diet; Holoptelea integrifolia; Inflammation; Iron; Lipids; Lipopolysaccharides; Magnesium; Nutrients; Nutrition; Original Article; Oxidants; Oxidizing agents; Pericarp; Protein deficiency; Protein sources; Proteins; Seeds; Single electrons; Tumor necrosis factor-α
• ISSN: 0022-1155; 0975-8402
• DOI: 10.1007/s13197-024-05930-4

Exploring unconventional protein sources can be an alternative strategy to meet the deficiency. The seeds of Chirabilva ( Holoptelea integrifolia Roxb., Family- Ulmaceae) are eaten raw by the ethnic communities of Southeast Asian countries. The present study assessed the chemical, nutritional, and biological potential of the seeds (HIS) and pericarp (HISP) of H. integrifolia. The seeds contain mainly fixed and very few essential oils. The fixed oil of HIS is composed primarily of unsaturated oleic (47%) and saturated palmitic (37%) acids. The HIS are exceptional due to a high content of lipid (50%), protein (24%), carbohydrates (19%), fiber (4%), and anti-nutritional components within permissible limits. The high content (in mg/Kg) of phosphorus (6000), magnesium (422), Calcium (279), and essential nutrients (Ni, Co, Zn, Fe, Cu, Mn, and Cr) in the range of (0.04–6.69) were observed. The moderate anti-oxidant potential of HISP was evident in single electron transfer in-vitro assays. Moreover, HISP extract and HIS solvent-extracted fixed oil showed anti-inflammatory action in lipopolysaccharide-induced HaCaT cells by significantly attenuating pro-inflammatory cytokines (TNF-α) without causing cytotoxicity. Results support de-oiled HIS cake as an alternative source of a high-protein diet and its oil with anti-inflammatory attributes for topical applications.