Time profiles of energy balance in dairy cows in association with metabolic status, inflammatory status, and disease

• Published in: Journal of dairy science 2024-11, Vol.107 (11), p.9960-9977
• Main Authors: Ma, J., Kok, A., Burgers, E.E.A., Bruckmaier, R.M., Goselink, R.M.A., Gross, J.J., Kemp, B., Lam, T.J.G.M., Minuti, A., Saccenti, E., Trevisi, E., Vossebeld, F., van Knegsel, A.T.M.
• Format: Article
• Language: English
• Subjects: Animals; Cattle; Cattle Diseases - blood; Cattle Diseases - metabolism; clustering; Energy Metabolism; Fatty Acids, Nonesterified - blood; Female; health; inflammation; Inflammation - metabolism; Inflammation - veterinary; Lactation; Milk - metabolism; negative energy balance; Oxidative Stress
• ISSN: 0022-0302; 1525-3198; 1525-3198
• DOI: 10.3168/jds.2024-24680

The early lactation period in dairy cows is characterized by complex interactions among energy balance (EB), disease, and alterations in metabolic and inflammatory status. The objective of this study was to cluster cows based on EB time profiles in early lactation and investigate the association between EB clusters and inflammatory status, metabolic status, oxidative stress, and disease. Holstein-Friesian dairy cows (n = 153) were selected and monitored for disease treatments during wk 1 to 6 in lactation. Weekly EB was calculated based on energy intake and energy requirements for maintenance and milk yield in wk 1 to 6 in lactation. Weekly plasma samples were analyzed for metabolic variables in wk 1 to 6, and inflammatory and oxidative stress variables in wk 1, 2, and 4 in lactation. Liver activity index (LAI) was computed from plasma albumin, cholesterol, and retino-binding protein concentration. First, cows were clustered based on time profiles of EB, resulting in 4 clusters (SP: stable positive; MN: mild negative; IN: intermediate negative; SN: severe negative). Cows in the SN cluster had higher plasma nonesterified fatty acids and BHB concentrations, compared with cows in the SP cluster, with the MN and IN clusters being intermediate. Cows in the SN cluster had a higher milk yield, lower DMI in wk 1, lower insulin concentration compared with cows in the SP cluster, and lower glucose and IGF-1 concentration compared with cows in the SP and MN clusters. Energy balance clusters were not related to plasma haptoglobin, cholesterol, albumin, paraoxonase, and LAI. Second, cows were grouped based on health status: IHP, cows with treatment for inflammatory health problem (endometritis, fever, clinical mastitis, vaginal discharge or retained placenta); OHP, cows with no IHP but treatment for other health problem (milk fever, cystic ovaries, claw and leg problems, rumen and intestine problems, or other diseases); and NHP, cows with no treatments, in the first 6 wk after calving. Energy balance was not different among health status groups. The IHP cows had lower nonesterified fatty acids and greater insulin concentration in plasma compared with OHP cows. The IHP cows had lower plasma albumin concentration, lower LAI, and higher haptoglobin concentration compared with OHP and NHP. Overall, EB time profiles were associated with the metabolic status of dairy cows in early lactation, but were only limitedly related to markers of inflammation and oxidative stress stat