The MKK3 MAPK cascade integrates temperature and after-ripening signals to modulate seed germination

• Published in: Proceedings of the National Academy of Sciences - PNAS 2024-07, Vol.121 (28), p.e2404887121
• Main Authors: Otani, Masahiko, Tojo, Ryo, Regnard, Sarah, Zheng, Lipeng, Hoshi, Takumi, Ohmori, Suzuha, Tachibana, Natsuki, Sano, Tomohiro, Koshimizu, Shizuka, Ichimura, Kazuya, Colcombet, Jean, Kawakami, Naoto
• Format: Article
• Language: English
• Subjects: Arabidopsis - genetics; Arabidopsis - growth & development; Arabidopsis - metabolism; Arabidopsis Proteins - genetics; Arabidopsis Proteins - metabolism; Gene Expression Regulation, Plant; Germination; MAP Kinase Kinase 3 - genetics; MAP Kinase Kinase 3 - metabolism; MAP Kinase Signaling System - physiology; Mitogen-Activated Protein Kinases - genetics; Mitogen-Activated Protein Kinases - metabolism; Plant Dormancy - genetics; Plant Dormancy - physiology; Seeds - genetics; Seeds - growth & development; Seeds - metabolism; Signal Transduction; Temperature
• ISSN: 0027-8424; 1091-6490; 1091-6490
• DOI: 10.1073/pnas.2404887121

The timing of seed germination is controlled by the combination of internal dormancy and external factors. Temperature is a major environmental factor for seed germination. The permissive temperature range for germination is narrow in dormant seeds and expands during after-ripening (AR) (dormancy release). Quantitative trait loci analyses of preharvest sprouting in cereals have revealed that MKK3, a mitogen-activated protein kinase (MAPK) cascade protein, is a negative regulator of grain dormancy. Here, we show that the MAPKKK19/20-MKK3-MPK1/2/7/14 cascade modulates the germination temperature range in seeds by elevating the germinability of the seeds at sub- and supraoptimal temperatures. The expression of and is induced around optimal temperature for germination in after-ripened seeds but repressed in dormant seeds. MPK7 activation depends on the expression levels of , with expression occurring under conditions permissive for germination. Abscisic acid (ABA) and gibberellin (GA) are two major phytohormones which are involved in germination control. Activation of the MKK3 cascade represses ABA biosynthesis enzyme gene expression and induces expression of ABA catabolic enzyme and GA biosynthesis enzyme genes, resulting in expansion of the germinable temperature range. Our data demonstrate that the MKK3 cascade integrates temperature and AR signals to phytohormone metabolism and seed germination.