Experimental and molecular modelling demonstration of effective DNA and protein binding as well as anticancer potential of two mononuclear Cu(II) and Co(II) complexes with isothiocyanate and azide as anionic residues

• Published in: International journal of biological macromolecules 2024-08, Vol.275 (Pt 2), p.133716, Article 133716
• Main Authors: Satapathi, Dibyendu, Das, Manik, Das, Uttam Kumar, Laha, Soumik, Kundu, Pronab, Choudhuri, Indranil, Bhattacharya, Nandan, Samanta, Bidhan Chandra, Chattopadhyay, Nitin, Maity, Tithi
• Format: Article
• Language: English
• Subjects: Ancillary ligand; Anticancer; DNA; HSA binding; Nuclear staining
• ISSN: 0141-8130; 1879-0003; 1879-0003
• DOI: 10.1016/j.ijbiomac.2024.133716

In the present study, one mononuclear Cu(II) [CuL(SCN)] (1) and one mononuclear Co(II) [CoLN3] (2) complexes, with a Schiff base ligand (HL) formed by condensation of 2-picolylamine and salicylaldehyde, have been successfully developed and structurally characterized. The square planer geometry of both complexes is fulfilled by the coordination of one deprotonated ligand and one ancillary ligand SCN−(1) or N3−(2) to the metal centre. Binding affinities of both complexes with deoxyribonucleic acid (DNA) and human serum albumin (HSA) are investigated using several biophysical and spectroscopic techniques. High values of the macromolecule-complex binding constants and other results confirm the effectiveness of both complexes towards binding with DNA and HSA. The determined values of the thermodynamic parameters support spontaneous interactions of both complexes with HSA, while fluorescence displacement and DNA melting studies establish groove-binding interactions with DNA for both complexes 1 and 2. The molecular modelling study validates the experimental findings. Both complexes are subjected to an MTT test establishing the anticancer property of complex 1 with lower risk to normal cells, confirmed by the IC50 values of the complex for HeLa cancer cells and HEK normal cells. Finally, a nuclear staining analysis reveals that the complexes have caused apoptotic cell death. Exploration of two newly synthesized Cu(II) and Co(II) molecular complexes with isothiocyanate and azide ancillary moiety respectively towards DNA/HSA binding and anticancer activity. [Display omitted]