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Synthetic Amine Linkers for Efficient Sortagging
Enzymatic site-specific bioconjugation techniques, in particular sortase-mediated ligation, are increasingly used to generate conjugated proteins for a wide array of applications. Extension of the utility and practicality of sortagging for diverse purposes is critically dependent on further improvem...
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| Published in: | Bioconjugate chemistry 2024-08, Vol.35 (8), p.1172-1181 |
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| Main Authors: | , , , , , , , , , , |
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| Language: | English |
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| container_end_page | 1181 |
| container_issue | 8 |
| container_start_page | 1172 |
| container_title | Bioconjugate chemistry |
| container_volume | 35 |
| creator | Bondarchuk, Tetiana Vaskiv, Diana Zhuravel, Elena Shyshlyk, Oleh Hrynyshyn, Yevhenii Nedialko, Oleksandr Pokholenko, Oleksandr Pohribna, Alla Kuchuk, Olga Brovarets, Volodymyr Zozulya, Sergey |
| description | Enzymatic site-specific bioconjugation techniques, in particular sortase-mediated ligation, are increasingly used to generate conjugated proteins for a wide array of applications. Extension of the utility and practicality of sortagging for diverse purposes is critically dependent on further improvement of the efficiency of sortagging reactions with a wider structural variety of substrates. We present a comprehensive comparative mass spectrometry screening study of synthetic nonpeptidic incoming amine nucleophile substrates of Staphylococcus aureus Sortase A enzyme. We have identified the optimal structural motifs among the chemically diverse set of 452 model primary and secondary amine-containing sortagging substrates, and we demonstrate the utility of representative amine linkers for efficient C-terminal biotinylation of nanobodies. |
| doi_str_mv | 10.1021/acs.bioconjchem.4c00143 |
| format | article |
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| identifier | ISSN: 1043-1802 |
| ispartof | Bioconjugate chemistry, 2024-08, Vol.35 (8), p.1172-1181 |
| issn | 1043-1802 1520-4812 1520-4812 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_3079174433 |
| source | American Chemical Society:Jisc Collections:American Chemical Society Read & Publish Agreement 2022-2024 (Reading list) |
| subjects | Amines - chemistry Aminoacyltransferases - metabolism Bacterial Proteins - chemistry Biotinylation Cysteine Endopeptidases - chemistry Cysteine Endopeptidases - metabolism Mass Spectrometry Mass spectroscopy Nanobodies Nucleophiles Single-Domain Antibodies - chemistry Sortase Staphylococcus aureus - enzymology Substrate Specificity Substrates |
| title | Synthetic Amine Linkers for Efficient Sortagging |
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