Biological and Physicochemical Analysis of Sr-Doped Hydroxyapatite/Chitosan Composite Layers

• Published in: Polymers 2024-07, Vol.16 (13), p.1922
• Main Authors: Zarif, Maria Elena, Bita, Bogdan, Yehia-Alexe, Sasa Alexandra, Negut, Irina, Gradisteanu Pircalabioru, Gratiela, Andronescu, Ecaterina, Groza, Andreea
• Format: Article
• Language: English
• Subjects: Absorption spectra; Antimicrobial agents; Banded structure; Biocompatibility; Biological effects; Bones; Chitosan; Composite materials; Fibroblasts; Fourier transforms; Hydroxyapatite; Infrared spectroscopy; Laser beam heating; Lasers; Magnetic properties; Magnetron sputtering; Microchannels; Morphology; Nitrates; Photoelectrons; Plasma; Pulsed lasers; Strontium; Substrates; Surface roughness; Temperature dependence; Tissue engineering; Wavelengths; X ray photoelectron spectroscopy
• ISSN: 2073-4360; 2073-4360
• DOI: 10.3390/polym16131922

In this work results are presented on the evaluation of HAp, HApSr, HAp_CS, and HApSr_CS layers deposited on Ti substrates regarding L929 cell viability and cytotoxicity as well as antimicrobial activity against , in connection with their physicochemical properties. The HAp and HApSr layers generated by radio-frequency magnetron sputtering technique were further covered with chitosan by a matrix-assisted pulsed laser evaporation technique. During the plasma depositions, the Ti substrates were heated externally by a home-made oven above 100 °C. The HApSr_CS layers generated on the unpolished Ti substrates at 100 °C and 400 °C showed the highest biocompatibility properties and antimicrobial activity against . The morphology of the layer surfaces, revealed by scanning electron microscopy, is dependent on substrate temperature and substrate surface roughness. The optically polished surfaces of Ti substrates revealed grain-like and microchannel structure morphologies of the layers deposited at 25 °C substrate temperature and 400 °C, respectively. Chitosan has no major influence on HAp and HApSr layer surface morphologies. X-ray photoelectron spectroscopy indicated the presence of Ca 2p peak characteristic of the HAp structure even in the case of the HApSr_CS samples generated at a 400 °C substrate temperature. Fourier transform infrared spectroscopy investigations showed shifts in the wavenumber positions of the P-O absorption bands as a function of Sr or chitosan presence in the HAp layers generated at 25, 100, and 400 °C substrate temperatures.