Regional Heterogeneity in Intestinal Epithelial Barrier Permeability and Mesenteric Perfusion After Thoracic Spinal Cord Injury

• Published in: Digestive diseases and sciences 2024-09, Vol.69 (9), p.3236-3248
• Main Authors: Radler, Jackson B., McBride, Amanda R., Saha, Kushal, Nighot, Prashant, Holmes, Gregory M.
• Format: Article
• Language: English
• Subjects: Animals; Biochemistry; Colon; Colon - blood supply; Colon - metabolism; Disease Models, Animal; Duodenum - metabolism; Gastroenterology; Hepatology; Intestinal Mucosa - metabolism; Male; Medicine; Medicine & Public Health; Occludin - metabolism; Oncology; Original Article; Oxidative stress; Oxidative Stress - physiology; Pathophysiology; Permeability; Proteins; Rats; Rats, Sprague-Dawley; Small intestine; Spinal cord injuries; Spinal Cord Injuries - metabolism; Spinal Cord Injuries - physiopathology; Thoracic Vertebrae; Tight Junctions - metabolism; Transplant Surgery
• ISSN: 0163-2116; 1573-2568; 1573-2568
• DOI: 10.1007/s10620-024-08537-z

Background Spinal cord injury (SCI) disrupts intestinal barrier function, thereby increasing antigen permeation and leading to poor outcomes. Despite the intestinal tract’s anatomic and physiologic heterogeneity, studies following SCI have not comprehensively addressed intestinal pathophysiology with regional specificity. Aims and Methods We used an experimental model of high thoracic SCI to investigate (1) regional mucosal oxidative stress using dihydroethidium labeling; (2) regional paracellular permeability to small- and large-molecular probes via Ussing chamber; (3) regional intestinal tight junction (TJ) protein expression; and (4) hindgut perfusion via the caudal mesenteric artery. Results Dihydroethidium staining was significantly elevated within duodenal mucosa at 3-day post-SCI. Molar flux of [ 14 C]-urea was significantly elevated in duodenum and proximal colon at 3-day post-SCI, while molar flux of [ 3 H]-inulin was significantly elevated only in duodenum at 3-day post-SCI. Barrier permeability was mirrored by a significant increase in the expression of pore-forming TJ protein claudin-2 in duodenum and proximal colon at 3-day post-SCI. Claudin-2 expression remained significantly elevated in proximal colon at 3-week post-SCI. Expression of the barrier-forming TJ protein occludin was significantly reduced in duodenum at 3-day post-SCI. Caudal mesenteric artery flow was unchanged by SCI at 3 days or 3 weeks despite significant reductions in mean arterial pressure. Conclusion These data show that T3-SCI provokes elevated mucosal oxidative stress, altered expression of TJ proteins, and elevated intestinal barrier permeability in the proximal intestine. In contrast, mucosal oxidative stress and intestinal barrier permeability were unchanged in the hindgut after SCI. This regional heterogeneity may result from differential sensitivity to reduced mesenteric perfusion, though further studies are required to establish a causal link. Understanding regional differences in intestinal pathophysiology is essential for developing effective treatments and standards of care for individuals with SCI.