Fission yeast Pib2 localizes to vacuolar membranes and regulates TOR complex 1 through evolutionarily conserved domains

TOR complex 1 (TORC1) is a multi‐protein kinase complex that coordinates cellular growth with environmental cues. Recent studies have identified Pib2 as a critical activator of TORC1 in budding yeast. Here, we show that loss of Pib2 causes severe growth defects in fission yeast cells, particularly w...

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Bibliographic Details
Published in:FEBS letters 2024-12, Vol.598 (23), p.2886-2896
Main Authors: Morozumi, Yuichi, Hayashi, Yumi, Chu, Cuong Minh, Sofyantoro, Fajar, Akikusa, Yutaka, Fukuda, Tomoyuki, Shiozaki, Kazuhiro
Format: Article
Language:English
Subjects:
Conserved Sequence
Evolution, Molecular
fission yeast
Intracellular Membranes - metabolism
Mechanistic Target of Rapamycin Complex 1 - genetics
Mechanistic Target of Rapamycin Complex 1 - metabolism
Mutation
Pib2
Protein Domains
Schizosaccharomyces - genetics
Schizosaccharomyces - metabolism
Schizosaccharomyces pombe Proteins - genetics
Schizosaccharomyces pombe Proteins - metabolism
TOR complex 1
vacuolar membrane
Vacuoles - metabolism
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Summary:TOR complex 1 (TORC1) is a multi‐protein kinase complex that coordinates cellular growth with environmental cues. Recent studies have identified Pib2 as a critical activator of TORC1 in budding yeast. Here, we show that loss of Pib2 causes severe growth defects in fission yeast cells, particularly when basal TORC1 activity is diminished by hypomorphic mutations in tor2, the gene encoding the catalytic subunit of TORC1. Consistently, TORC1 activity is significantly compromised in the tor2 hypomorphic mutants lacking Pib2. Moreover, as in budding yeast, fission yeast Pib2 localizes to vacuolar membranes via its FYVE domain, with its tail motif indispensable for TORC1 activation. These results strongly suggest that Pib2‐mediated positive regulation of TORC1 is evolutionarily conserved between the two yeast species. Budding yeast Pib2, whose FYVE domain and tail motif are conserved in its fission yeast ortholog, was previously characterized as an activator of TORC1, a master regulator of cell growth and metabolisms. This study has demonstrated the conserved function of Pib2 in fission yeast; Pib2 localizes to vacuolar membranes through its FYVE domain and positively regulates TORC1.
ISSN:0014-5793
1873-3468
1873-3468
DOI:10.1002/1873-3468.14980