A novel quantitative double antigen sandwich ELISA for detecting total antibodies against Candida albicans enolase 1

Purpose This study aimed to develop a double antigen sandwich ELISA (DAgS-ELISA) method for more efficient, accurate, and quantitative detection of total antibodies against Candida albicans enolase1 (CaEno1) for diagnosing invasive candidiasis (IC). Methods DAgS-ELISA was developed using recombinant...

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Bibliographic Details
Published in:European journal of clinical microbiology & infectious diseases 2024-09, Vol.43 (9), p.1815-1823
Main Authors: Wang, Lihui, He, Zongzhong, Guo, Yiyang, Ran, Xiangyang, Cheng, Yan, He, Zhengxin
Format: Article
Language:English
Subjects:
Antibodies
Antigens
Bacterial diseases
Biomedical and Life Sciences
Biomedicine
Candida albicans
Candidiasis
Internal Medicine
Medical Microbiology
Monoclonal antibodies
Performance evaluation
Phosphopyruvate hydratase
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Summary:Purpose This study aimed to develop a double antigen sandwich ELISA (DAgS-ELISA) method for more efficient, accurate, and quantitative detection of total antibodies against Candida albicans enolase1 (CaEno1) for diagnosing invasive candidiasis (IC). Methods DAgS-ELISA was developed using recombinant CaEno1 and a monoclonal antibody as the standard. Performance evaluation included limit of detection, accuracy, and repeatability. Dynamic changes in antibody levels against CaEno1 in serum from systemic candidiasis mice were analyzed using DAgS-ELISA. Patient serum samples from IC, Candida colonization, bacterial infections, and healthy controls were analyzed with DAgS-ELISA and indirect ELISA. Results DAgS-ELISA outperformed indirect ELISA in terms of linear range and test background. In systemic candidiasis mice, a distinctive ‘double-peak’ pattern in dynamic antibody levels was observed. Additionally, there was a high level of consistency in the positive rates of CaEno1 antibodies detected by both DAgS-ELISA and indirect ELISA. While the positivity rates differed among patient groups, no significant variations in antibody levels were detected among the various positive patient groups. Conclusions DAgS-ELISA offers a reliable novel approach for IC diagnosis, enabling rapid, accurate, and quantitative detection of CaEno1 antibodies. Further validation and optimization are needed for its clinical application and effectiveness.
ISSN:0934-9723
1435-4373
1435-4373
DOI:10.1007/s10096-024-04899-4