WenTongGanPi decoction alleviates diarrhea-predominant irritable bowel syndrome by improving intestinal barrier

• Published in: Journal of ethnopharmacology 2024-11, Vol.334, p.118544, Article 118544
• Main Authors: Li, Yaoguang, Chen, Yangyang, Liao, Zhengyue, Liu, Yixin, Liu, Chenhao, Yang, Wenjing, Bai, Jing, Huang, Xinggui, Hao, Yule, Liu, Sijing, Liu, Yi
• Format: Article
• Language: English
• Subjects: Animals; Diarrhea; Diarrhea - drug therapy; Disease Models, Animal; Drugs, Chinese Herbal - pharmacology; Drugs, Chinese Herbal - therapeutic use; Gut microbiota; Intestinal barrier; Intestinal Mucosa - drug effects; Intestinal Mucosa - metabolism; Irritable bowel syndrome; Irritable Bowel Syndrome - drug therapy; Male; MAPK signal pathway; Molecular Docking Simulation; Permeability; Rats; Rats, Sprague-Dawley; WenTongGanPi decoction
• ISSN: 0378-8741; 1872-7573; 1872-7573
• DOI: 10.1016/j.jep.2024.118544

WenTongGanPi Decoction (WTGPD) is a representative medical practice of the Fuyang School of Traditional Chinese Medicine (TCM), which originated from the classical Lu's Guizhi method. WTGPD places emphasis on the balance and functionality of yang qi, and is effective in treating TCM symptoms related to liver qi stagnation and spleen yang deficiency. In TCM, diarrhea-predominant irritable bowel syndrome (IBS-D) is often diagnosed as liver depression and spleen deficiency, and the use of WTGPD has shown significant therapeutic effect. However, the underlying mechanism of WTGPD treating IBS-D remains unclear. To explore the effect and mechanism of WTGPD in the treatment of IBS-D. An IBS-D model with liver depression and spleen deficiency was constructed by chronic immobilization stress stimulation and sennae folium aqueous gavage. The impact of WTGPD on IBS-D rats was evaluated through measurements of body weight, fecal water content, and abdominal withdrawal reflex (AWR). Intestinal permeability was assessed using hematoxylin-eosin (HE), alcian blue-periodic acid schiff (AB-PAS), immunofluorescence (IF) staining, and quantitative real-time PCR (qRT-PCR). The components of WTGPD were analyzed using UPLC-Q-TOF-MS. The underlying mechanisms were investigated through network pharmacology, transcriptomics sequencing, western blot (WB), molecular docking, and 16S rRNA sequencing. WTGPD treatment effectively alleviated diarrhea and abnormal pain in IBS-D rats (P