Cell wall polysaccharide enhances Lacticaseibacillus paracasei strain Shirota growth in milk and contributes to acid and bile tolerance

• Published in: International journal of food microbiology 2024-09, Vol.422, p.110811, Article 110811
• Main Authors: Kato, Kosuke, Serata, Masaki, Nakamura, Madoka, Ando, Minoru, Suzuki, Tomo, Okumura, Takekazu
• Format: Article
• Language: English
• Subjects: Acids - pharmacology; Animals; Bile - metabolism; Bile Acids and Salts - pharmacology; Cattle; Cell Wall - metabolism; Cell wall polysaccharide; Fermentation; Gastrointestinal stress; Hydrogen-Ion Concentration; Lactic acid bacteria; Lacticaseibacillus paracasei; Lacticaseibacillus paracasei - metabolism; Microbial Viability; Milk - microbiology; Polysaccharides - metabolism; Polysaccharides - pharmacology; Probiotics; Probiotics - pharmacology; Soy Milk; Survivability
• ISSN: 0168-1605; 1879-3460; 1879-3460
• DOI: 10.1016/j.ijfoodmicro.2024.110811

LCPS-1, a cell wall polysaccharide (CWPS), is bound to the cell wall of the probiotic Lacticaseibacillus paracasei (formerly known as Lactobacillus casei) strain Shirota (LcS). Generally, the role of CWPS in the viability and survivability of bacteria is yet to be fully understood. This study aimed to elucidate the role of LCPS-1 in the viability and survivability of LcS. A mutant strain completely lacking LCPS-1 was constructed and evaluated for growth in bovine and soy milk and susceptibility to acid and bile. The growth of the mutant in bovine and soy milk temporarily stalled after the late logarithmic phase while wild-type LcS continued growing, resulting in a significantly lower number of viable cells for the mutant strain (p