Nanozyme-Catalyzed Colorimetric Detection of the Total Antioxidant Capacity in Body Fluids by Paper-Based Microfluidic Chips

Total antioxidants play a crucial role in human health, and detection of the total antioxidant capacity (TAC) has broad application prospects in fields such as food safety, environmental assessment, and disease diagnosis. However, a long detection time, cumbersome steps, high cost, reliance on profe...

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Bibliographic Details
Published in:ACS applied materials & interfaces 2024-07, Vol.16 (30), p.39857-39866
Main Authors: Wu, Hongjiao, Chen, Jiaqi, Lin, Pengcheng, Su, Yiqian, Li, Huiqin, Xiao, Wei, Peng, Jianhui
Format: Article
Language:English
Subjects:
antioxidant activity
blood
colorimetry
detection limit
disease diagnosis
environmental assessment
food safety
Functional Nanostructured Materials (including low-D carbon)
human health
mobile telephones
organ-on-a-chip
photography
point-of-care systems
quantitative analysis
saliva
sweat
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Summary:Total antioxidants play a crucial role in human health, and detection of the total antioxidant capacity (TAC) has broad application prospects in fields such as food safety, environmental assessment, and disease diagnosis. However, a long detection time, cumbersome steps, high cost, reliance on professional equipment, and nonportability still remain significant challenges. In this work, an efficient strategy of point-of-care testing (POCT) of the TAC in body fluids by nanozyme-catalyzed colorimetric paper-based microfluidic sensors is proposed. The paper-based microfluidic sensors coupled with a smartphone can reduce testing costs and provide portability. The nanozyme prepared by the solvothermal method presents Michaelis constants of 0.11 and 0.129 mM for H2O2 and TMB, respectively. A method for immobilizing nanozymes and chromogenic agents on a paper-based microfluidic chip is established. Based on smartphone photography and image grayscale extraction, the TAC can be qualitatively detected with a detection limit and linear range of 33.4 and 50–700 μM, respectively. Furthermore, the proposed sensor can realize the one-step quantitative analysis of the TAC in body fluids (blood, saliva, and sweat) within 15 min. The proposed nanozyme-catalyzed colorimetric paper-based microfluidic sensors presented in this study exhibit promising application prospects in the fields of biochemical analysis and POCT.
ISSN:1944-8244
1944-8252
1944-8252
DOI:10.1021/acsami.4c07835