Exploration of GmDof11-lncRNA13082 Module Regulating Oil Synthesis in Plants

• Published in: Journal of agricultural and food chemistry 2024-07, Vol.72 (30), p.16889-16899
• Main Authors: Zhao, Qiuzhu, Zhao, Dingyi, Wang, Yashuo, Li, Yuxin, Ni, Chang, Su, Zitong, Lian, Panhang, Liu, Shuying, Liu, Huijing, Zhang, Jun, Yao, Dan
• Format: Article
• Language: English
• Subjects: Arabidopsis - genetics; Arabidopsis - metabolism; Biotechnology and Biological Transformations; Fatty Acids - biosynthesis; Fatty Acids - metabolism; Gene Expression Regulation, Plant; Glycine max - genetics; Glycine max - metabolism; Plant Proteins - genetics; Plant Proteins - metabolism; Plants, Genetically Modified - genetics; Plants, Genetically Modified - metabolism; RNA, Long Noncoding - genetics; RNA, Long Noncoding - metabolism; Seeds - chemistry; Seeds - genetics; Seeds - metabolism; Soybean Oil - genetics; Soybean Oil - metabolism; Transcription Factors - genetics; Transcription Factors - metabolism
• ISSN: 0021-8561; 1520-5118; 1520-5118
• DOI: 10.1021/acs.jafc.4c03084

Soybean (Glycine max [Linn.] Merr.) is an important oilseed crop. Although transcription factors (TFs) can coordinate the expression of mRNA and lncRNA, their coordination in the soybean oil synthesis pathway remains unclear. This study examined the interaction between the TF GmDof11 and lncRNA13082 and found that overexpression of GmDof11 led to an increase in the number of Arabidopsis seeds, thousand seed weight, crude protein, hydrolysis amino acid, and soluble sugar. Additionally, it reduced the triglyceride and starch contents and affected the proportion of fatty acids, increasing the contents of palmitic acid, stearic acid, and linolenic acid. The yeast two-hybrid experiments revealed that GmDof11 interacts with GmBCCP1, GmLEC1b, and GmFAB2 proteins. In the RT-qPCR analysis of transgenic soybean roots, it was found that GmDof11 can activate the production of lncRNA13082 and work in conjunction with lncRNA13082 to oversee oil synthesis and nutrient storage. Our research provides robust theoretical evidence for a comprehensive resolution of TF-lncRNA regulation in the soybean oil synthesis network.