Green light mediates atypical photomorphogenesis by dual modulation of Arabidopsis phytochromes B and A

• Published in: Journal of integrative plant biology 2024-09, Vol.66 (9), p.1915-1933
• Main Authors: Xu, Miqi, Wang, Yi‐Yuan, Wu, Yujie, Zhou, Xiuhong, Shan, Ziyan, Tao, Kunying, Qian, Kaiqiang, Wang, Xuncheng, Li, Jian, Wu, Qingqing, Deng, Xing Wang, Ling, Jun‐Jie
• Format: Article
• Language: English
• Subjects: Arabidopsis - genetics; Arabidopsis - metabolism; Arabidopsis - radiation effects; Arabidopsis Proteins - genetics; Arabidopsis Proteins - metabolism; Cotyledon - genetics; Cotyledon - metabolism; Cotyledon - radiation effects; cotyledon development; Fluence; Gene Expression Regulation, Plant - radiation effects; Green Light; Hypocotyl - growth & development; Hypocotyl - metabolism; Hypocotyl - radiation effects; Hypocotyls; Light; photobody; Photomorphogenesis; phytochrome; Phytochrome A - genetics; Phytochrome A - metabolism; Phytochrome B; Phytochrome B - genetics; Phytochrome B - metabolism; Phytochromes; PIF3; Regulations; Seedlings; Seedlings - genetics; Seedlings - growth & development; Seedlings - metabolism; Seedlings - radiation effects
• ISSN: 1672-9072; 1744-7909; 1744-7909
• DOI: 10.1111/jipb.13742

ABSTRACT Although green light (GL) is located in the middle of the visible light spectrum and regulates a series of plant developmental processes, the mechanism by which it regulates seedling development is largely unknown. In this study, we demonstrated that GL promotes atypical photomorphogenesis in Arabidopsis thaliana via the dual regulations of phytochrome B (phyB) and phyA. Although the Pr‐to‐Pfr conversion rates of phyB and phyA under GL were lower than those under red light (RL) in a fluence rate‐dependent and time‐dependent manner, long‐term treatment with GL induced high Pfr/Pr ratios of phyB and phyA. Moreover, GL induced the formation of numerous small phyB photobodies in the nucleus, resulting in atypical photomorphogenesis, with smaller cotyledon opening angles and longer hypocotyls in seedlings compared to RL. The abundance of phyA significantly decreased after short‐ and long‐term GL treatments. We determined that four major PHYTOCHROME‐INTERACTING FACTORs (PIFs: PIF1, PIF3, PIF4, and PIF5) act downstream of phyB in GL‐mediated cotyledon opening. In addition, GL plays opposite roles in regulating different PIFs. For example, under continuous GL, the protein levels of all PIFs decreased, whereas the transcript levels of PIF4 and PIF5 strongly increased compared with dark treatment. Taken together, our work provides a detailed molecular framework for understanding the role of the antagonistic regulations of phyB and phyA in GL‐mediated atypical photomorphogenesis. Green light regulates atypical photomorphogenesis in Arabidopsis thaliana via the dual regulations of phytochromes B and A. Although green light activates phyB and phyA, green light retards protein body formation of phyB in the nucleus and decreases the protein levels of phyA.