Point-of-care testing of Pseudomonas aeruginosa using PCN-222(Pt) prepared by nanoconfinement-guided protocol to catalyze gas generation reaction

• Published in: Analytica chimica acta 2024-08, Vol.1317, p.342892, Article 342892
• Main Authors: Li, Jizhou, Yuan, Hongwei, Gao, Xinyue, Fu, Zhifeng
• Format: Article
• Language: English
• Subjects: Catalysis; Humans; Hydrogel pellets; Hydrogen Peroxide - chemistry; Metal Nanoparticles - chemistry; Metal-Organic Frameworks - chemistry; Palladium - chemistry; PCN-222(Pt); Phage; Platinum - chemistry; Point-of-Care Testing; Porphyrins - chemistry; Pressure sensor; Pseudomonas aeruginosa; Pseudomonas aeruginosa - isolation & purification; Zirconium - chemistry
• ISSN: 0003-2670; 1873-4324; 1873-4324
• DOI: 10.1016/j.aca.2024.342892

Pathogenic bacteria are keeping threatening global public health since they can cause many infectious diseases. The traditional microorganism identification and molecular diagnostic techniques are insufficiently sensitive, time-consuming, or expensive. Thus it is of great interest to establish pressure signal-based sensing platforms for point-of-care testing of pathogenic bacteria to achieve timely diagnosis of infectious diseases. Rational design and synthesis of nano-sized probes with high peroxidase-mimicking activity have been a long-term cherished goal for improving the sensitivity of pressure signal-based sensing methods. Guided by nanoconfinement effect, PCN-222(Pt) was prepared by confining Pt clusters within the channels of a zirconium porphyrin MOFs material termed as PCN-222. In comparison to regular platinum nanoparticles, palladium@platinum core-shell nanodendrites, and platinum-coated gold nanoparticles, the prepared PCN-222(Pt) displayed superior peroxidase-mimicking activity with outstanding efficiency for catalyzing the decay of H2O2 to produce O2. Thus it was used as a pressure signal probe to establish a sensitive method on a hydrogel pellets platform for analyzing Pseudomonas aeruginosa (P. aeruginosa), for which polymyxin B and a phage termed as JZ1 were used as recognition agents for the target pathogen. P. aeruginosa was quantified with a handheld pressure meter within a broad range of 2.2 × 102–2.2 × 107 cfu mL−1. This method was used to quantify P. aeruginosa in various biological and food samples with acceptable accuracy and reliability. The proposed nanoconfinement-guided protocol provides a novel approach for rational design and preparation of nano-sized probes with high peroxidase-mimicking activity for catalyzing gas-generation reaction. Thus this study opens an avenue for establishment of sensitive pressure signal-based sensing methods for pathogenic bacteria, which shows broad application prospects in medical diagnosis of infectious diseases. PCN-222(Pt) with outstanding peroxidase-mimicking activity for catalyzing gas generation reaction was prepared with a nanoconfinement-guided protocol, and used as a pressure probe to establish a sensitive method on a hydrogel pellets platform for analyzing Pseudomonas aeruginosa. [Display omitted] •PCN-222(Pt) was prepared by a nanoconfinement-guided protocol.•PCN-222(Pt) shows outstanding activity for catalyzing gas generation reaction.•PCN-222(Pt) was used as a pressure probe for anal