PagKNAT2/6b regulates tension wood formation and gravitropism by targeting cytokinin metabolism

Abstract Tension wood is a specialized xylem tissue associated with gravitropism in angiosperm trees. However, few regulators of tension wood formation have been identified. The molecular mechanisms underpinning tension wood formation remain elusive. Here, we report that a Populus KNOTTED-like homeo...

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Bibliographic Details
Published in:Tree physiology 2024-08, Vol.44 (8)
Main Authors: Hu, Mengxuan, Zhao, Shutang, Zhao, Yanqiu, Lu, Mengzhu, Song, Xueqin
Format: Article
Language:English
Subjects:
Cytokinins - metabolism
Gene Expression Regulation, Plant
Gravitropism - physiology
Homeodomain Proteins - genetics
Homeodomain Proteins - metabolism
Plant Proteins - genetics
Plant Proteins - metabolism
Plants, Genetically Modified - genetics
Populus - genetics
Populus - growth & development
Populus - metabolism
Populus - physiology
Wood - growth & development
Wood - physiology
Xylem - genetics
Xylem - growth & development
Xylem - metabolism
Xylem - physiology
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Summary:Abstract Tension wood is a specialized xylem tissue associated with gravitropism in angiosperm trees. However, few regulators of tension wood formation have been identified. The molecular mechanisms underpinning tension wood formation remain elusive. Here, we report that a Populus KNOTTED-like homeobox gene, PagKNAT2/6b, is involved in tension wood formation and gravity response. Transgenic poplar plants overexpressing PagKNAT2/6b displayed more sensitive gravitropism than controls, as indicated by increased stem curvature. Microscopic examination revealed greater abundance of fibre cells with a gelatinous cell wall layer (G-layer) and asymmetric growth of secondary xylem in PagKNAT2/6b overexpression lines. Conversely, PagKNAT2/6b dominant repression plants exhibited decreased tension wood formation and reduced response to gravity stimulation. Moreover, sensitivity to gravity stimulation showed a negative relationship with development stage. Expression of genes related to growth and senescence was affected in PagKNAT2/6b transgenic plants. More importantly, transcription activation and electrophoretic mobility shift assays suggested that PagKNAT2/6b promotes the expression of cytokinin metabolism genes. Consistently, cytokinin content was increased in PagKNAT2/6b overexpression plants. Therefore, PagKNAT2/6b is involved in gravitropism and tension wood formation, likely via modulation of cytokinin metabolism.
ISSN:1758-4469
1758-4469
DOI:10.1093/treephys/tpae090