The first study of the prevalence and genetic diversity of Theileria equi and Babesia caballi in horses in Russia

• Published in: Parasitology research (1987) 2024-07, Vol.123 (7), p.279, Article 279
• Main Authors: Rar, Vera, Marchenko, Victor, Suntsova, Olga, Epikhina, Tamara, Tikunov, Artem, Meltsov, Ivan, Fedorets, Valeria, Igolkina, Yana, Kozlova, Irina, Tikunova, Nina
• Format: Article
• Language: English
• Subjects: Animals; Babesia - classification; Babesia - genetics; Babesia - isolation & purification; Babesia caballi; Babesiosis - epidemiology; Babesiosis - parasitology; Biomedical and Life Sciences; Biomedicine; Conserved sequence; DNA, Protozoan - genetics; DNA, Ribosomal - chemistry; DNA, Ribosomal - genetics; Genetic diversity; Genetic Variation; Genotype; Genotype & phenotype; Genotypes; Horse Diseases - epidemiology; Horse Diseases - parasitology; Horses - parasitology; Immunology; Medical Microbiology; Microbiology; Pathogens; Phylogeny; Prevalence; RNA, Ribosomal, 18S - genetics; rRNA 18S; Russia - epidemiology; Sequence Analysis, DNA; Siberia - epidemiology; Theileria - classification; Theileria - genetics; Theileria - isolation & purification; Theileria equi; Theileriasis - epidemiology; Theileriasis - parasitology
• ISSN: 0932-0113; 1432-1955; 1432-1955
• DOI: 10.1007/s00436-024-08300-3

Equine piroplasmosis (EP) is a global worldwide infection, which can lead to the death of animals. Despite the causative agents of EP being well studied, there are no data on the distribution and genetic characteristics of EP agents in any region of Russia. In this study, blood samples from 750 horses from Novosibirsk province, Irkutsk province, and Altai region of Russian Siberia were examined for the presence of EP agents. Theileria equi and Babesia caballi were detected in all examined regions, with mean prevalence rates of 60.4% and 7.2%, respectively. The identified pathogens were genetically characterized by the 18S rRNA gene. The determined T. equi sequences were highly conserved and belonged to genotypes A and E, with genotype E being found in 88.6% of genotyped samples. In contrast to T. equi , B. caballi sequences were genetically diverse. Seven sequence variants of B. caballi were identified, and only two of them matched known sequences from the GenBank database. The determined B. caballi sequences belonged to four distinct branches within genotype A. Mixed infections with several variants of B. caballi or with T. equi and B. caballi were common. The conducted phylogenetic analysis based on all available B. caballi sequences of the 18S rRNA gene (> 900 bp) from GenBank and from this study first demonstrated the presence of five monophyletic clusters within genotype A and three clusters within genotype B. Thus, the genetic study of B. caballi from Siberia has significantly expanded the data on the genetic diversity of this pathogen.