Rab7 GTPase-Mediated stress signaling enhances salinity tolerance in AlRabring7 tobacco transgenics by modulating physio-biochemical parameters

• Published in: Plant physiology and biochemistry 2024-09, Vol.214, p.108928, Article 108928
• Main Authors: Agarwal, Parinita, Chittora, Anjali, Baraiya, Bhagirath M., Fatnani, Dhara, Patel, Khantika, Akhyani, Dhanvi D., Parida, Asish K., Agarwal, Pradeep K.
• Format: Article
• Language: English
• Subjects: ABA; AlRabring7; RING proteins; ROS homeostasis; Salinity stress; Stomata
• ISSN: 0981-9428; 1873-2690; 1873-2690
• DOI: 10.1016/j.plaphy.2024.108928

The RING-type E3 ligases play a significant role in stress signaling, primarily through post-translational regulation. Ubiquitination is a crucial post-translational modification that regulates the turnover and activity of proteins. The overexpression of AlRabring7, RING-HC E3 Ub ligase in tobacco provides insights into the regulation of salinity and ABA signaling in transgenic tobacco. The seed germination potential of AlRabring7 transgenics was higher than WT, with NaCl and ABA treatments. The transgenics showed improved morpho-physio-biochemical parameters in response to salinity and ABA treatments. The photosynthetic pigments, soluble sugars, reducing sugars and proline increased in transgenics in response to NaCl and ABA treatments. The decreased ROS accumulation in transgenics on NaCl and ABA treatments can be co-related to improved activity of enzymatic and non-enzymatic antioxidants. The potential of transgenics to maintain ABA levels with ABA treatment, highlights the active participation of ABA feedback loop mechanism. Interestingly, the ability of AlRabring7 transgenics to upregulate Rab7 protein, suggests its role in facilitating vacuolar transport. Furthermore, the improved potassium accumulation and reduced sodium content indicate an efficient ion regulation mechanism in transgenic plants facilitating higher stomatal opening. The expression of downstream ion transporter (NbNHX1 and NbVHA1), ABA signaling (NbABI2 and NbABI5) and vesicle trafficking (NbMON1) responsive genes were upregulated with stress. The present study, reports that AlRabring7 participates in maintaining vacuolar transport, ion balance, ROS homeostasis, stomatal regulation through activation of Rab7 protein and regulation of downstream stress-responsive during stress. This emphasizes the potential of AlRabring7 gene for improved performance and resilience in challenging environments. •AlRabring7 is involved in Rab7 GTPase-mediated regulation of intracellular membrane trafficking pathways.•AlRabring7 tobacco transgenics showed improved morpho-physio-biochemical parameters, antioxidant enzymes activity and stomatal regulation under salt stress.•An active ABA feedback loop is maintained in transgenics for stress tolerance.•The transgenics showed upregulation of stress responsive downstream genes.