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RNA interference of the clock gene period disrupts circadian rhythms in the expression of genes related to insecticide resistance in the chagas disease vector Triatoma infestans (Hemiptera: Reduviidae)
•The silencing of per gene in T. infestans abolished its canonical rhythm of expression at transcriptional level.•The effect of silencing the gene per showed the loss of rhythmicity in the expression profiles of CPR and CYP4EM7 genes.•The effect of silencing the gene per demonstrated the biological...
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Published in: | Acta tropica 2024-09, Vol.257, p.107329, Article 107329 |
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creator | Córdoba, L.E. Pérez de Rosas, A.R. García, B.A. Serradell, M.del C. Remón, C. Mougabure-Cueto, G.A. Stroppa, M.M. |
description | •The silencing of per gene in T. infestans abolished its canonical rhythm of expression at transcriptional level.•The effect of silencing the gene per showed the loss of rhythmicity in the expression profiles of CPR and CYP4EM7 genes.•The effect of silencing the gene per demonstrated the biological clock involvement in the regulation of CPR and CYP4EM7 genes expression.
In Triatoma infestans it was observed pyrethroid resistance attributed in part to an elevated oxidative metabolism mediated by cytochromes P450. The nicotinamide adenine dinucleotide phosphate (NADPH) cytochrome P450 reductase (CPR) plays a crucial role in catalysing the electron transfer from NADPH to all cytochrome P450s. The daily variations in the expression of CPR gene and a P450 gene (CYP4EM7), both associated with insecticide resistance, suggested that their expressions would be under the endogenous clock control. To clarify the involvement of the clock in orchestration of the daily fluctuations in CPR and CYP4M7 genes expression, it was proposed to investigate the effect of silencing the clock gene period (per) by RNA interference (RNAi). The results obtained allowed to establish that the silencing of per gene was influenced by intake schemes used in the interference protocols. The silencing of per gene in T. infestans reduced its expression at all the time points analysed and abolished the characteristic rhythm in the transcriptional expression of per mRNA. The effect of the per gene silencing in the expression profiles at the transcriptional level of CPR and CYP4EM7 genes showed the loss of rhythmicity and demonstrated the biological clock involvement in the regulation of t
heir expression.
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doi_str_mv | 10.1016/j.actatropica.2024.107329 |
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In Triatoma infestans it was observed pyrethroid resistance attributed in part to an elevated oxidative metabolism mediated by cytochromes P450. The nicotinamide adenine dinucleotide phosphate (NADPH) cytochrome P450 reductase (CPR) plays a crucial role in catalysing the electron transfer from NADPH to all cytochrome P450s. The daily variations in the expression of CPR gene and a P450 gene (CYP4EM7), both associated with insecticide resistance, suggested that their expressions would be under the endogenous clock control. To clarify the involvement of the clock in orchestration of the daily fluctuations in CPR and CYP4M7 genes expression, it was proposed to investigate the effect of silencing the clock gene period (per) by RNA interference (RNAi). The results obtained allowed to establish that the silencing of per gene was influenced by intake schemes used in the interference protocols. The silencing of per gene in T. infestans reduced its expression at all the time points analysed and abolished the characteristic rhythm in the transcriptional expression of per mRNA. The effect of the per gene silencing in the expression profiles at the transcriptional level of CPR and CYP4EM7 genes showed the loss of rhythmicity and demonstrated the biological clock involvement in the regulation of t
heir expression.
[Display omitted]</description><identifier>ISSN: 0001-706X</identifier><identifier>ISSN: 1873-6254</identifier><identifier>EISSN: 1873-6254</identifier><identifier>DOI: 10.1016/j.actatropica.2024.107329</identifier><identifier>PMID: 39033969</identifier><language>eng</language><publisher>Netherlands: Elsevier B.V</publisher><subject>Animals ; Chagas' disease ; Circadian Rhythm - genetics ; Clock regulation ; CYP4EM7 and CPR genes rhythms ; Cytochrome P-450 Enzyme System - genetics ; Disease Vectors ; Gene Expression Regulation - drug effects ; Insect Proteins - genetics ; Insect Proteins - metabolism ; Insecticide Resistance - genetics ; NADPH-Ferrihemoprotein Reductase - genetics ; NADPH-Ferrihemoprotein Reductase - metabolism ; Period gene silencing ; Pyrethroid metabolism ; RNA Interference ; Triatoma - drug effects ; Triatoma - genetics ; Triatoma infestans</subject><ispartof>Acta tropica, 2024-09, Vol.257, p.107329, Article 107329</ispartof><rights>2024 Elsevier B.V.</rights><rights>Copyright © 2024 Elsevier B.V. All rights reserved.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><cites>FETCH-LOGICAL-c251t-e4e1efa07a4dbb7f21153e4a5f0c03f932130d971828554f9b8498469387ae1e3</cites><orcidid>0009-0008-3395-2776 ; 0000-0003-2148-6030</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27924,27925</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/39033969$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Córdoba, L.E.</creatorcontrib><creatorcontrib>Pérez de Rosas, A.R.</creatorcontrib><creatorcontrib>García, B.A.</creatorcontrib><creatorcontrib>Serradell, M.del C.</creatorcontrib><creatorcontrib>Remón, C.</creatorcontrib><creatorcontrib>Mougabure-Cueto, G.A.</creatorcontrib><creatorcontrib>Stroppa, M.M.</creatorcontrib><title>RNA interference of the clock gene period disrupts circadian rhythms in the expression of genes related to insecticide resistance in the chagas disease vector Triatoma infestans (Hemiptera: Reduviidae)</title><title>Acta tropica</title><addtitle>Acta Trop</addtitle><description>•The silencing of per gene in T. infestans abolished its canonical rhythm of expression at transcriptional level.•The effect of silencing the gene per showed the loss of rhythmicity in the expression profiles of CPR and CYP4EM7 genes.•The effect of silencing the gene per demonstrated the biological clock involvement in the regulation of CPR and CYP4EM7 genes expression.
In Triatoma infestans it was observed pyrethroid resistance attributed in part to an elevated oxidative metabolism mediated by cytochromes P450. The nicotinamide adenine dinucleotide phosphate (NADPH) cytochrome P450 reductase (CPR) plays a crucial role in catalysing the electron transfer from NADPH to all cytochrome P450s. The daily variations in the expression of CPR gene and a P450 gene (CYP4EM7), both associated with insecticide resistance, suggested that their expressions would be under the endogenous clock control. To clarify the involvement of the clock in orchestration of the daily fluctuations in CPR and CYP4M7 genes expression, it was proposed to investigate the effect of silencing the clock gene period (per) by RNA interference (RNAi). The results obtained allowed to establish that the silencing of per gene was influenced by intake schemes used in the interference protocols. The silencing of per gene in T. infestans reduced its expression at all the time points analysed and abolished the characteristic rhythm in the transcriptional expression of per mRNA. The effect of the per gene silencing in the expression profiles at the transcriptional level of CPR and CYP4EM7 genes showed the loss of rhythmicity and demonstrated the biological clock involvement in the regulation of t
heir expression.
[Display omitted]</description><subject>Animals</subject><subject>Chagas' disease</subject><subject>Circadian Rhythm - genetics</subject><subject>Clock regulation</subject><subject>CYP4EM7 and CPR genes rhythms</subject><subject>Cytochrome P-450 Enzyme System - genetics</subject><subject>Disease Vectors</subject><subject>Gene Expression Regulation - drug effects</subject><subject>Insect Proteins - genetics</subject><subject>Insect Proteins - metabolism</subject><subject>Insecticide Resistance - genetics</subject><subject>NADPH-Ferrihemoprotein Reductase - genetics</subject><subject>NADPH-Ferrihemoprotein Reductase - metabolism</subject><subject>Period gene silencing</subject><subject>Pyrethroid metabolism</subject><subject>RNA Interference</subject><subject>Triatoma - drug effects</subject><subject>Triatoma - genetics</subject><subject>Triatoma infestans</subject><issn>0001-706X</issn><issn>1873-6254</issn><issn>1873-6254</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2024</creationdate><recordtype>article</recordtype><recordid>eNqNkc1uEzEUhS0EoqHwCsjsymKCf-aXXRUBrVSBVBWJnXVjXzcOM-PB9kT0EXkrPE1ALFlZvvrOOdc-hLzhbM0Zr9_t16ATpOAnp2EtmCjzvJGie0JWvG1kUYuqfEpWjDFeNKz-dkZexLjPN9FU4jk5kx2Tsqu7Ffl1-_mSujFhsBhw1Ei9pWmHVPdef6f3OCKdMDhvqHExzFOKVLugwTgYadg9pN0Qs8GjBn9OAWN0flxcFm2kAXtIaGjymYqok9POYB5HFxMsgSex3sE9xCUFISI9ZNQHehccJD9ApiwugkgvrnBwU94Y3tNbNPPBOQP49iV5ZqGP-Op0npOvHz_cba6Kmy-frjeXN4UWFU8FlsjRAmugNNttYwXnlcQSKss0k7aTgktmuoa3oq2q0nbbtuzasu5k20CWynNycfSdgv8x553U4KLGvocR_RyVZG32aGTZZrQ7ojr4GANaNQU3QHhQnKmlSbVX_zSplibVscmsfX2KmbcDmr_KP9VlYHMEMD_24DCoqN3SoHEh_50y3v1HzG-IbLne</recordid><startdate>202409</startdate><enddate>202409</enddate><creator>Córdoba, L.E.</creator><creator>Pérez de Rosas, A.R.</creator><creator>García, B.A.</creator><creator>Serradell, M.del C.</creator><creator>Remón, C.</creator><creator>Mougabure-Cueto, G.A.</creator><creator>Stroppa, M.M.</creator><general>Elsevier B.V</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><orcidid>https://orcid.org/0009-0008-3395-2776</orcidid><orcidid>https://orcid.org/0000-0003-2148-6030</orcidid></search><sort><creationdate>202409</creationdate><title>RNA interference of the clock gene period disrupts circadian rhythms in the expression of genes related to insecticide resistance in the chagas disease vector Triatoma infestans (Hemiptera: Reduviidae)</title><author>Córdoba, L.E. ; Pérez de Rosas, A.R. ; García, B.A. ; Serradell, M.del C. ; Remón, C. ; Mougabure-Cueto, G.A. ; Stroppa, M.M.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c251t-e4e1efa07a4dbb7f21153e4a5f0c03f932130d971828554f9b8498469387ae1e3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2024</creationdate><topic>Animals</topic><topic>Chagas' disease</topic><topic>Circadian Rhythm - genetics</topic><topic>Clock regulation</topic><topic>CYP4EM7 and CPR genes rhythms</topic><topic>Cytochrome P-450 Enzyme System - genetics</topic><topic>Disease Vectors</topic><topic>Gene Expression Regulation - drug effects</topic><topic>Insect Proteins - genetics</topic><topic>Insect Proteins - metabolism</topic><topic>Insecticide Resistance - genetics</topic><topic>NADPH-Ferrihemoprotein Reductase - genetics</topic><topic>NADPH-Ferrihemoprotein Reductase - metabolism</topic><topic>Period gene silencing</topic><topic>Pyrethroid metabolism</topic><topic>RNA Interference</topic><topic>Triatoma - drug effects</topic><topic>Triatoma - genetics</topic><topic>Triatoma infestans</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Córdoba, L.E.</creatorcontrib><creatorcontrib>Pérez de Rosas, A.R.</creatorcontrib><creatorcontrib>García, B.A.</creatorcontrib><creatorcontrib>Serradell, M.del C.</creatorcontrib><creatorcontrib>Remón, C.</creatorcontrib><creatorcontrib>Mougabure-Cueto, G.A.</creatorcontrib><creatorcontrib>Stroppa, M.M.</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Acta tropica</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Córdoba, L.E.</au><au>Pérez de Rosas, A.R.</au><au>García, B.A.</au><au>Serradell, M.del C.</au><au>Remón, C.</au><au>Mougabure-Cueto, G.A.</au><au>Stroppa, M.M.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>RNA interference of the clock gene period disrupts circadian rhythms in the expression of genes related to insecticide resistance in the chagas disease vector Triatoma infestans (Hemiptera: Reduviidae)</atitle><jtitle>Acta tropica</jtitle><addtitle>Acta Trop</addtitle><date>2024-09</date><risdate>2024</risdate><volume>257</volume><spage>107329</spage><pages>107329-</pages><artnum>107329</artnum><issn>0001-706X</issn><issn>1873-6254</issn><eissn>1873-6254</eissn><abstract>•The silencing of per gene in T. infestans abolished its canonical rhythm of expression at transcriptional level.•The effect of silencing the gene per showed the loss of rhythmicity in the expression profiles of CPR and CYP4EM7 genes.•The effect of silencing the gene per demonstrated the biological clock involvement in the regulation of CPR and CYP4EM7 genes expression.
In Triatoma infestans it was observed pyrethroid resistance attributed in part to an elevated oxidative metabolism mediated by cytochromes P450. The nicotinamide adenine dinucleotide phosphate (NADPH) cytochrome P450 reductase (CPR) plays a crucial role in catalysing the electron transfer from NADPH to all cytochrome P450s. The daily variations in the expression of CPR gene and a P450 gene (CYP4EM7), both associated with insecticide resistance, suggested that their expressions would be under the endogenous clock control. To clarify the involvement of the clock in orchestration of the daily fluctuations in CPR and CYP4M7 genes expression, it was proposed to investigate the effect of silencing the clock gene period (per) by RNA interference (RNAi). The results obtained allowed to establish that the silencing of per gene was influenced by intake schemes used in the interference protocols. The silencing of per gene in T. infestans reduced its expression at all the time points analysed and abolished the characteristic rhythm in the transcriptional expression of per mRNA. The effect of the per gene silencing in the expression profiles at the transcriptional level of CPR and CYP4EM7 genes showed the loss of rhythmicity and demonstrated the biological clock involvement in the regulation of t
heir expression.
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subjects | Animals Chagas' disease Circadian Rhythm - genetics Clock regulation CYP4EM7 and CPR genes rhythms Cytochrome P-450 Enzyme System - genetics Disease Vectors Gene Expression Regulation - drug effects Insect Proteins - genetics Insect Proteins - metabolism Insecticide Resistance - genetics NADPH-Ferrihemoprotein Reductase - genetics NADPH-Ferrihemoprotein Reductase - metabolism Period gene silencing Pyrethroid metabolism RNA Interference Triatoma - drug effects Triatoma - genetics Triatoma infestans |
title | RNA interference of the clock gene period disrupts circadian rhythms in the expression of genes related to insecticide resistance in the chagas disease vector Triatoma infestans (Hemiptera: Reduviidae) |
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